Methods in cell biology : prepared under the auspices of the American Society for Cell Biology.v. 78 /edited by M. Bishr Omary, Piere A. Coulombe. --Elsevier, 2004. -- (58.1574/M592/v.78)

Contents

Contents:

1.       Isolation, Characterization, and in Vitro Assembly of Intermediate Filaments  3

2.       Intermediate Filament Protein Structure Determination  25

3.       Intracellular Microrheology as a Tool for the Measurement of the Local Mechanical Properties of Live Cells  46

4.       Developing Mouse Models to Study Intermediate Filament Function  65

5.       Uncovering the Roles of Intermediate Filaments in Apoptosis  96

6.       Molecular Genetics Methods for Human Intermediate Filament Diseases  132

7.       Intermediate Filaments and Multiparameter Flow Cytometry for the Study of Solid Tumors  163

8.       Intermediate Filament Proteins Inclusions  206

9.       Stress Models for the Study of Intermediate Filament Function  230

10.   Regulation of Intermediate Filament Gene Expression  267

11.   Fluorescence-Based Methods for Studying Intermediate Filaments  298

12.   Imaging of Keratin Dynamics during the Cell Cycle and in Response to Phosphatase Inhibition  322

13.   Approaches to Study Phosphorylation of Intermediate Filament Proteins Using Site-Specific and Phosphorylation State-Specific Antibodies  354

14.   Approaches to Study Posttranslational R Egulation of Intermediate Filament Proteins  374

15.   Hair Keratins and Hair Follicle-Specific Epithelial Keratins  414

16.   Skin: An Ideal Model System to Study Keratin Genes and Proteins  454

17.   Studying Simple Epithelial Keratins in Cells and Tissues  489

18.   Mucle Intermediate Filament Proteins  519

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