Forensic DNA typing protocols / edited by Angel Carracedo. -- (Methods in molecular biology ; v. 297). -- Humana Press, 2005 (58.17/M592/v.297)

Contents:

1. Forensic DNA-Typing Technologies: a Review  1

2. DNA Extraction and Quantitation of Forensic Samples Using the Phenol-Chloroform Method and Real-Time PCR  13

3. A Real-Time PCR Protocol to Determine the Number of amelogenine (X-Y) Gene Copies From forensic DNA Samples  31

4. Species Determination: the Role and Use of the Cytochrome b Gene  45

5. Constructing STR Mulitplex Assays  53

6. Y Chromosome STR Typing  67

7. Using Online Databases for Developing SNP Markers of Forensic Interest  83

8. SNP Typing in Forensic Genetics: A Review  107

9. Genotyping SNPs With the LightCycler  127

10. Universal Tag Arrays in forensic SNP analysis  141

11. SNP Typing on the NanoChip electronic Microarray  155

12. Genotyping SNPs Using a UV-Photocleavable Oligonucleotide in MALDI-TOF MS  169

13. Mitochondrial D-Loop and coding Sequence Analysis Using Pyrosequencing  179

14. SNaPshot Typing of Mitochondrial DNA Coding Region Variants  197

15. Typing of Y Chromosome SNPs With Multiplex PCR Methods  209

16. Y Chromosome SNP analysis Using single-Base Extension: a Hierarchical Multiplex Design  229

17. SNaPshot for Pharmacogenetics by Ninisequencing  243

18. Methods for the Study of ancient DNA  253

19. Protocols for ancient DNA Typing  265