Adoptive immunotherapy : methods and protocols / edited by Burkhard Ludewig and Matthias W. Hoffmann. -- (Methods in molecular medicine ; v. 109). -- Humana Press, 2005, (63.472/A241)

Contents:

1. Exploiting Dendritic Cells for Active Immunotherapy of Cancer and Chronic Infection  1

2. A Mathematical Approach for Optimizing Dendritic Cell-Based Immunotherapy  19

3. Delivery of Tumor antigens to Dendritic Cells using Biodegradable Microspheres  35

4. RNA Transfection of Dendritic Cells  47

5. Isolation and Generation of clinical-Grade Dendritic Cells Using the ClinicMACS System  55

6. Generation of Clinical Grade Monocyte-Derived Dendritic Cells Using the CliniMACS System  71

7. Adenoviral Transduction of Dendritic Cells  83

8. Generation of Autologous Peptide and Protein-Pulsed Dendritic Cells for Patient-Specific Immunotherapy  97

9. Phenotypical and Functional Characterization of Clinical-Grade Dendritic Cells  113

10. Dendritic Cells in Clinical Trials for Multiple Myeloma  127

11. Identification of Tumor-Associated Autoantigens With SEREX  137

12. Application of Proteomics and Protein Analysis for Biomarker and Taget finding for Immunotherapy  155

13. Isolation and Expansion of Tumor-Reactive Cytotoxic T-Cell Clones for Adoptive Immunotherapy  175

14. Tracking Adoptively Transferred Antigen-Specific T-Cells With Peptide/MHC Multimers  185

15. Gene Transfer of MHC-Restricted Receptors  201

16. Immunotherapy With CTL Restricted by Nonself MHC  215

17. Designing TCR for Cancer Immunotherapy  229

18. Isolation and Expansion of Tumor-specific CD4+ T-Cells by Neans of Cytokine Secretion  257

19. Methods for the Ex Vivo Characterization of Human CD8+ T Subsets Based on Gene Expression and Replicative History analysis  265

20. Regulator T Cells in antitumor therapy: Isolation and functional Testing of CD4+CD25+ Regulatory T-Cells  285

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