Cell cycle control : mechanism and protocols / edited by tim Humphrey, gavin Brooks. -- (Methods in molecular biology ; v. 296). -- Humana press, 2005. -- (58.17/M592/v.296)

Contents:

1. Cell Cycle molecules and Mechanisms of the Budding and Fission Yeasts  3

2, The Plant Cell Cycle: an Overview  31

3. The C. elegans Cell Cycle: Overview of Molecules and Mechanisms  51

4. Developmental Control of Growth and Cell cycle Progression in Drosophila  69

5. The Xenopus Cell Cycle: an Overview  95

6. The Mammalian Cell Cycle: An Overview  113

7. Synchronization of Cell Populations in G1/S and G2/M Phases of the Cell Cycle  157

8. Mapping Origins of DNA Replication in Eukaryotes  167

9. In Situ Assay for Analyzing the Chromatin Binding of Proteins In fission Yeast  181

10. Application of Magnetic Beads to Purify Cells Transiently Transfected With Plasmids Encoding short Hairpin RNAs  189

11. The Cell Cycle and Virus Infection  197

12. Methods for Preparation of Proteins and Protein Complexes That Regulate the Eukaryotic Cell Cycle for Structural Studies  219

13. E2F Transcription Factors and pRb Pocket Proteins in Cell Cycle Progression  239

14. Forkhead (FOX) Transcription Factors and the Cell Cycle: Measurement of DNA Binding by FoxO and FoxM Transcription Factors  247

15. measurement of Geminin Activity in Xenopus Egg Extracts  263

16. CDK-Activating Kinases: Detection and Activity Measurements  279

17. Cyclins, cyclin-Dependent Kinases, and Cyclin-Dependent Kinase Inhibitors: Detection Methods and Activity Measurements  291

18. Measurement of Wee Kinase Activity  299

19. CDC25 Dual-Specificity Protein Phosphatases: Detection and Activity Measurements  329

20. Assaying Cell Cycle Checkpoints: Activity of the protein Kinase Chk1  345

21. Polo-Like Kinase-1: Activity Measurement and RNAi-Mediated Knockdown  355

22. The IpI1/Aurora Kinase family: Methods of Inhibition and functional analysis in Mammalian Cells  371

23. Purification of the Ndc80 Kinetochore subcomplex from Xenopus Eggs  383