Immunochemical protocols / edited by Robert Burns. -- 3rd ed. -- (Methods in molecular biology ; v. 295). -- Humana Press, 2005. -- (58.17/M592/v.295)

Contents:

1. Immunization Strategies for Antibody Production  1

2. Synthetic Peptides as Antigens for Antibody Production  13

3. Production of Polyclonal Antibodies in Rabbits  27

4. Making Hybridomas  41

5. Growing Hybridomas  55

6. Phage Display Vectors for the In Vitro Generation fo Human Antibody Fragments  71

7. Purification of antibodies and Preparation of antibody Fragments  97

8. Conjugation of Antibodies to alkaline Phosphatase  123

9. Conjugation of Antibodies to Horseradish Peroxidase  127

10. Conjugation of antibodies to fluorescein or Rhodamine  131

11. Conjugation fo Antibodies to Biotin  135

12. preparation of Colloidal Gold Probes  155

13. Immunogold Probes in Lighe Microscopy  173

14. Immunoelectron Microscope Techniques in Plant Virus Diagnosis  193

15. Dual-antibody Sandwich Enzyme-Linked Immunosorbent Assay  207

16. Competitive Enzyme-Linked Immunosorbent Assay  215

17. Immunoblotting Techniques  227

18. Epitope Mapping  255

19. Generation and Testing of Immonoaffinity Columns  269

20. Immunocapture-Polymerase Chain Reaction  281

21. Magnetic Cell sorting  291

22. Cell Enrichment and Immunochemical Staining  301