Analytical techniques for the elucidation of protein function / edited by Isao Suetake, Rohit Sharma, Hironobu Hojo. -- Hoboken, NJ : John Wiley & Sons, 2023. – (58.17421/A532)

Contents

Preface  /x
Editor's Biographies  xi
List of Contributors  xiii
1      EPR Spectroscopy  1
1.1     Outline of EPR Spectroscopy  2
1.2     Biological Applications of EPR  13
2      Introduction to Incoherent Neutron Scattering: A Powerful Technique to Investigate the Dynamics of Bio-macromolecules
2.1     Introduction  39
2.2     Basic Theory and Dynamical Information Obtained from iNS  42
2.3     Examples of Biological Applications of iNS  58
2.4     Summary  63
3      Elucidation of Protein Function Using Raman Spectroscopy  69
3.1     Introduction  69
3.2     Basic Principle and Working of Raman Spectroscopy  71
3.3     Advances in Raman Spectroscopy Techniques  74
3.4     Applications  91
3.5     Conclusion  92
4      Fundamental Principles of Impedance Spectroscopy and its Biological Applications  101
4.1     Introduction  101
4.2     Biological Applications of Impedance Spectroscopy  113
4.3     Conclusion  119
5      Mass Spectrometry Imaging  125
5.1     Introduction  125
5.2     Workflow of MSI  126
5.3     Mass Microscope  128
5.4     Visualization of Small Molecules (Pharmaceutical)  128
5.5     Structural Isomer Discrimination Imaging (Steroid Hormones)  130
5.6     Visualization of Proteins (Intact, Digestion)  133
5.7     Visualization of Protein Function (Enzymatic Activity Visualization)  134
5.8     Summary  139
6      Elucidation of Protein Function Using Single-molecule Monitoring by Quantum Dots  143
6.1     Introduction  143
6.2     Synthesis Methods  148
6.3     Bioconjugation  151
6.4     Analytical Methods for Single-molecule Monitoring by Quantum Dots  152
6.5     Applications  156
6.6     Limitations of QDs  163
6.7     Conclusion  163
7      Biological Solid-state NMR Spectroscopy  169
7.1     Introduction  169
7.2     Magnetic Interactions for NMR  170
7.3     Methods for Solid-state NMR  173
7.4     Applications of Solid-state NMR to Biological Molecular Systems  180
7.5     Concluding Remarks  184
8      Etectrical.ly Induced Bubble Knife and Its Applications  191
8.1     Introduction  191
8.2     Electrically Induced Bubble Knife  192
8.3     Electrically Induced Bubble Injector  199
8.4     Plasma-induced Bubble Injector  201
8.5     Protein Crystallization by Electrically Induced Bubbles  202
8.6     Protein Crystallization by Plasma-induced Bubbles  207
Index  215