Brain development : methods and protocols / edited by Simon G. Sprecher. -- 2nd ed. -- Totowa, NJ, United States : Humana Press, 2020. – (58.17/M592/v.2047)

Contents

Preface
Contributors
PART I INVERTEBRATE MODELS
1 Combining BrdU-Labeling to Detection of Neuronal Markers to Monitor Adult Neurogenesis in Hydra
2 Reverse Genetic Approaches to Investigate the Neurobiology of the Cnidarian Sea Anemone Nematostella vectensis
3 Generating Transgenic Reporter Lines for Studying Nervous System Development in the Cnidarian Nematostella vectensis
4 Immunostaining and In Situ Hybridization of the Developing Acoel Nervous System
5  Immunostaining of the Embryonic and Larval Drosophila Brain
6 Nonfluorescent RNA In Situ Hybridization Combined with Antibody Staining to Visualize Multiple Gene Expression Patterns in the Embryonic Brain of Drosophila
7 Analysis of Complete Neuroblast Cell Lineages in the Drosophila Embryonic Brain via DiI Labeling
8 Flybow to Dissect Circuit Assembly in the Drosophila Brain: An Update
9  Live Cell Imaging of Neural Stem Cells in the Drosophila Larval Brain
10 CRISPR/Cas9 Genome Editing to Study Nervous System Development in Drosophila
11 The Red Flour Beetle as Model for Comparative Neural Development: Genome Editing to Mark Neural Cells in Tribolium Brain Development
12 A Protocol for Double Fluorescent In Situ Hybridization and Immunohistochemistry for the Study of Embryonic Brain Development in Tribolium castaneum
13 Immunohistochemistry and Fluorescent Whole Mount RNA In Situ Hybridization in Larval and Adult Brains of Tribolium
14 X-Ray Microscopy of the Larval Crustacean Brain
15  Immunolocalization of Neurotransmitters and Neuromodulators in the Developing Crayfish Brain
16 Immunostainings in Nervous System Development of the Nematode C. elegans
17 Methods in Brain Development of Molluscs
18 A Simple Method to Identify Ascidian Brain Lineage Cells at Neural Plate Stages Following In Situ Hybridization
19  Spawning Induction and Embryo Micromanipulation Protocol in the Amphioxus Branchiostoma lanceolatum
PART II  VERTEBRATE MODELS
20  In Situ Hybridization and Immunostaining of Xenopus Brain
21  Morpholino Studies in Xenopus Brain Development
22 Sensitive Multiplexed Fluorescent In Situ Hybridization Using Enhanced Tyramide Signal Amplification and Its Combination with Immunofluorescent Protein Visualization in Zebrafish
23  Live Morphometric Classification of Sensory Neurons in Larval Zebrafish
24 Immunohistochemistry and In Situ Hybridization in the Developing Chicken Brain
25 Gene Silencing in Chicken Brain Development
26 Transplantation of Neural Tissue: Quail-Chick Chimeras
27 Immunohistochemistry and RNA In Situ Hybridization in Mouse Brain Development
28 The Cre/Lox System to Assess the Development of the Mouse Brain
29 In Utero Electroporation to Study Mouse Brain Development