Plant chemical genomics : methods and protocols / edited by Glenn R. Hicks, Chunhua Zhang. -- Second edition. -- New York, NY : Springer, 2021. – (58.17/M592/v.2213)

Contents

Contributors
PART I  SCREENING FOR NOVEL SMALL MOLECULES
1  Chemical Genetics to Uncover Mechanisms Underlying Lipid-Mediated Signaling Events in Plants
2  Method for Phenotypic Chemical Screening to Identify Cryptochrome Inhibitors
3  Whole-Seedling-Based Chemical Genetic Screens in Arabidopsis
4  Identification of Type III Secretion Inhibitors for Plant Disease Management
5  Investigation of Drug Efficacy by Screening Bioactive Chemical Effects on Plant Cell Subcellular Architecture
PART II  New Approaches To VACATION: PEPTIDE SCREEN FOR NOVEL EFFECTORS
6  In Vivo Chemical Genomics with Random Cyclized Peptides
7  Interfering Peptides Targeting Protein-Protein Interactions in the Ethylene Plant Hormone Signaling Pathway as Tools to Delay Plant Senescence
PART III  CHEMICAL GENOMICS IN HORMONE SIGNALING
8  The Screening for Novel Inhibitors of Auxin-Induced Ca2+ Signaling
9  Identification of ABA Receptor Agonists Using a Multiplexed High-Throughput Chemical Screening
10  A Luciferase Reporter Assay to Identify Chemical Activators of ABA Signaling
11  Identification of Novel Molecular Regulators Modulating Ethylene Biosynthesis Using EMS-Based Genetic Screening
12  Investigation of Auxin Biosynthesis and Action Using Auxin Biosynthesis Inhibitors
PART IV  Talk GET IDENTIFICATION AND CONFIRMATION APPROACHES
13  Target Profiling of an Anticancer Drug Curcumin by an In Situ Chemical Proteomics Approach
14  Label-Free Target Identification and Confirmation Using Thermal Stability Shift Assays
15  Drug Affinity Responsive Target Stability (DARTS) Assay to Detect Interaction Between a Purified Protein and a Small Molecule
16  Using Differential Scanning Fluorimetry (DSF) to Detect Ligand Binding with Purified Protein
17  Microscale Thermophoresis (MST) to Detect the Interaction Between Purified Protein and Small Molecule
index