Chondrocytes : methods and protocols / edited by Tariq M. Haqqi, Véronique Lefebvre -- New York, NY : Humana Press, 2021. – (58.17/M592/v.2245)

Contents

Preface
Contributors
1  Isolation of Chondrocytes from Human Cartilage and Cultures in Monolayer and 3D
2  Protocol for the Isolation of Intact Chondrons from Healthy and Osteoarthritic Human Articular Cartilage
3  Cartilage-Specific Cre Recombinase Transgenes/Alleles in the Mouse
4  Isolation of Mouse Growth Plate and Articular Chondrocytes for Primary Cultures
5  Fetal Growth Plate Cartilage: Histological and Immunohistochemical Techniques
6  Preparation of Adult Mouse Skeletal Tissue Sections for RNA In Situ Hybridization
7  MicroRNA In Situ Hybridization in Paraffin-Embedded Human Articular Cartilage and Mouse Knee Joints
8  Laser Capture Microdissection of Mouse Growth Plate Cartilage
9  RNA Isolation from Articular Cartilage Tissue
10  Small Nucleolar RNA Expression Profiling in Cartilage
11  MicroRNA Expression Profiling, Target Identification, and Validation in Chondrocytes
12   ChIP-Seq Assays from Mammalian Cartilage and Chondrocytes
13   MicroRNAs and Regulation of Autophagy in Chondrocytes
14  Mitochondrial Biogenesis, Activity, and DNA Isolation in Chondrocytes
15  Assessing Chondrocyte Status by Immunofluorescence-Mediated Localization of Parkin Relative to Mitochondria
Index