Exocytosis and endocytosis : methods and protocols / edited by Florence Niedergang, Nicolas Vitale, Stéphane Gasman. -- New York, NY : Humana Press, 2021. – (58.17/M592/v.2233)

Contents

Preface
Contributors
PART I  ENDOCYTOSIS
1  Stoichiometry of Receptors at the Plasma Membrane During Their Endocytosis Using Total Internal Reflection Fluorescent (TIRF) Microscopy Live Imaging and Single-Molecule Tracking
2  Measuring Endocytosis During Proliferative Cell Quiescence
3  Measurements of Compensatory Endocytosis by Antibody Internalization and Quantification of Endocytic Vesicle Distribution in Adrenal Chromaffin Ceils
4  Quantitative Methods to Study Endocytosis and Retrograde Transport of Cargo Proteins
5  High-Content Drug-Discovery Screening of Endocytosis Pathways.
6  Methods for Monitoring Endocytosis in Astrocytes
7  Monitoring Activity-Dependent Bulk Endocytosis in Primary Neuronal Culture Using Large Fluorescent Dextrans
PART II  EXOCYTOSIS
8  Quantitative Flow Cytometry-Based Assays for Measuring Constitutive Secretion
9  High-Throughput Screening for Insulin Secretion Modulators
10  Different Approaches to Record Human Sperm Exocytosis
11  Bovine Chromaffin Cells: Culture and Fluorescence Assay for Secretion
12  Measurement of Exocytosis in Genetically Manipulated Mast Cells
13  Super-Resolution Microscopy and Particle-Tracking Approaches for the Study of Vesicular Trafficking in Primary Neutrophils
14  An Approach to Monitor Exocytosis in White Adipocytes
15  Amperometry in Single Cells and Tissue
16  Measurements of Exocytosis by Capacitance Recordings and Calcium Uncaging in Mouse Adrenal Chromaffin Cells
17  Retention Using Selective Hooks-Synchronized Secretion to Measure Local Exocytosis
18  Combining Single Molecule Super-Resolution Imaging Techniques to Unravel the Nanoscale Organization of the Presynapse
19  Induction of Ca2+-Dependent Exocytotic Processes by Laser Ablation of Endothelial Cells
20  Transmission Electron Microscopy and Tomography on Plasma Membrane Sheets to Study Secretory Docking
21  Spatial and Temporal Aspects of Exocytosis Studied on the Isolated Plasma Membranes
Correction to: High-Throughput Screening for Insulin Secretion Modulators
Index