Advanced methods in molecular biology and biotechnology : a practical lab manual / Khalid Z. Masoodi, Sameena Maqbool Lone and Rovidha Saba Rasool. -- London : Academic Press, 2021. – (58.178/M412)

Contents

Preface
Foreword
Acknowledgment
Introduction
About the author
Disclaimer
CHAPTER 1 Introduction to molecular biology techniques
CHAPTER 2 Agarose gel electrophoresis
CHAPTER 3 SDS-polyacrylamide gel electrophoresis
CHAPTER 4 Immunoelectrophoresis
CHAPTER 5 Plasmid DNA isolation using alkaline lysis method from E. coil DH5α cells
CHAPTER 6 Restriction digestion of plasmid DNA
CHAPTER 7 Genomic DNA extraction from the plant leaves using the CTAB method
CHAPTER 8 Genomic DNA extraction from silkworm using modified phenol: chloroform method (Suzuki et ah, 1972)
CHAPTER 9 Genomic DNA extraction from Fungal Mycelium using the modified Cenis, 1992 method
CHAPTER 10 Bacterial transformation using CaCl2
CHAPTER 11 Transformation of E. coil by electroporation
CHAPTER 12 Transfection of cells using lipofectamine
CHAPTER 13 Total RNA isolation
CHAPTER 14 RNA isolation from plant tissues with high phenolic compounds and polysaccharides
CHAPTER 15 Total protein extraction with TCA-acetone and 2D gel electrophoresis
CHAPTER 16 Dansyl-Edman method of peptide sequencing
CHAPTER 17 ISSR analysis
CHAPTER 18 Gene cloning/recombinant DNA technology
CHAPTER 19 Polymerase chain reaction (PCR)
CHAPTER 20 Blotting/hybridization and its types
CHAPTER 21 Dot blot analysis
CHAPTER 22 ELISA (enzyme-linked immunosorbent assay)
CHAPTER 23 Study of principle of centrifugation
CHAPTER 24 Study of principle of chromatography
CHAPTER 25 Thin layer chromatography (TLC)
CHAPTER 26 Gel-filtration or size-exclusion chromatography
CHAPTER 27 Ion-exchange chromatography
CHAPTER 28 To perform affinity chromatography
CHAPTER 29 To perform paper chromatography
CHAPTER 30 Growth of bacterial cultures and preparation of growth curve
CHAPTER 31 To study standard operating procedures (SOPs) for various equipment
Index