Total chemical synthesis of proteins / edited by Lei Liu, Ashraf Brik, Philip Dawson. -- Weinheim : Wiley-VCH, 2021. – (58.17421/T717)

Contents

Preface
1      Characterization of Protein Molecules Prepared by Total Chemical Synthesis
2      Automated Fast Flow Peptide Synthesis 17
3      N,S- and N,Se-Acyl Transfer Devices in Protein Synthesis 59
4      Chemical Synthesis of Proteins Through Native Chemical Ligation of Peptide Hydrazides 87
5      Expanding Native Chemical Ligation Methodology with Synthetic Amino Acid Derivatives  119
6      Peptide Ligations at Sterically Demanding Sites  161
7      Controlling Segment Solubility in Large Protein Synthesis 185
8      Toward HPLC-free Total Chemical Synthesis of Proteins  211
9      Solid-phase Chemical ligation  259
10     Ser/Thr Ligation for Protein Chemical Synthesis 285
11     Protein Semisynthesis 307
12     Bio-orthogonal Imine Chemistry in Chemical Protein Synthesis 327
13     Deciphering Protein Fording Using Chemical Protein Synthesis 357
14     Chemical Synthesis of Ubiquitinated Proteins for Biochemical Studies 383
15     Glycoprotein Synthesis 411
16     Chemical Synthesis of Membrane Proteins 437
17     Chemical Synthesis of Selenoproteins 463
18     Histone Synthesis 489
19     Application of Chemical Synthesis to Engineer Protein Backbone Connectivity
20     Beyond Phosphate Esters: Synthesis of Unusually phosphorylated Peptides and Proteins for Proteomic Research  533
21     Cyclic Peptides via Ligation Methods 553
Index 579