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An introduction to molecular biotechnology : fundamentals, methods and applications / edited by Michael Wink. -- Third, completely revised edition. -- Weinheim : Wiley-VCH, 2020. – (58.2/I61i/3rd ed.) |
Contents
Abbreviations xix
Part I Fundamentals of Cellular and Molecular Biology 1
1 The Cell as the Basic Unit of Life
2 Structure and Function of Cellular Macromolecules 9
3 Structure and Functions of a Cell 33
4 Biosynthesis and Function of Macromolecules (DNA, RNA, and Proteins) 63
5 Distributing Proteins in the Cell (Protein Sorting) 87
Part II Standard Methods in Molecular Biotechnology 103
7 Isolation and Purification of Proteins 105
8 Mass Spectrometry and Applications in Proteomics and Microbial Identification 117
9 Isolation of DNA and RNA 129
10 Chromatography and Electrophoresis of Nucleic Acids 133
11 Hybridization of Nucleic Acids 137
12 Use of Enzymes in the Modification of Nucleic Acids 141
13 Polymerase Chain Reaction 147
14 DNA Sequencing 153
15 Cloning Procedures 159
16 Expression of Recombinant Proteins 171
17 Patch Clamp Method 181
18 Cell Cycle Analysis 187
19 Microscopic Techniques 197
20 Laser Applications 209
Part III Key Topics 215
21 Sequencing the Universe of Life 217
22 Cellular Systems Biology 239
23 Protein-Protein and Protein-DNA Interactions 261
24 Bioinformatics 277
25 Drug Research 297
26 Drug Targeting and Prodrugs 311
27 Molecular Diagnostics in Medicine 323
28 Recombinant Antibodies and Phage Display 339
29 Genetically Modified Mice and Their Impact in Medical Research 361
30 Plant Biotechnology 375
31 Biocatalysis in the Chemical industry 393
Part IV Biotechnology Industry 411
32 Industrial Application: Biotech Industry, Markets, and Opportunities 413
33 Patents in the Molecular Biotechnology Industry: Legal and Ethical Issues 425
34 Drug Approval in the European Union and United States 435
35 Emergence of a Biotechnology Industry 445
36 The 101 of Founding a Biotech Company 453
37 Marketing 459
Glossary 463
Index 491
