Methods in stem cell biology. Part A / edited by Ilio Vitale, Gwenola Manic, Lorenzo Galluzzi. -- Cambridge, MA : Academic Press is an imprint of Elsevier, 2022. – (58.1574/M592/v.170)

Contents

Contributors
CHAPTER 1 0rthotopic brain tumor models derived from glioblastoma stem-like cells
               1. Introduction
               2. Preparing a GBM stem cell line from a surgical specimen
               3. Cell maintenance
               4. Implantation of GSCs
               5. Monitoring of tumor growth and treatment response
               Acknowledgments
               References
CHAPTER 2 identification and isolation of slow-cycling glioma stem cells
               1. Introduction
               2. Materials
               3. Methods
               4. Notes
               5. Concluding remarks
               Acknowledgments
               References
CHAPTER 3 Immunohistochemical analysis of the developing mouse cortex
               1. Introduction
               2. Materials and methods
               3. Conclusion
               4. Notes
               Acknowledgments
               References
CHAPTER 4 Subcellular fractionation of brain tumor stem cells
               1. Introduction
               2. Cell maintenance
               3. Subcellular fractionation
              4. Evaluation of subcellular fraction purity by Western blotting.
               5. Conclusion remarks
               6. Notes
              Acknowledgments
              References
CHAPTER 5 microRNAs transcriptional profiling of mammary stem cells isolated by PKH26 staining and FACS sorting
              1. Introduction
              2. Materials
              3. Methods
              4. Notes
              Acknowledgments
              Disclosures
              References
CHAPTER 6 Genome-wide RNA interference screen in cancer stem cells
              1. Introduction
              2. Global overview
              3. Preliminary steps
              4. Cell maintenance
              5. Collagen coating ,of culture plates
              6. Reverse transfection of siRNAs
              7. ALDEFLUOR assay
              8. Image acquisition and analysis
              9. Data pre-processing and candidate selection
             10. Notes
             Acknowledgments
             Disclosures
             References
CHAPTER 7 Clonal analysis of human clonogenic keratinocytes
               1. Introduction: The meaning and importance of the clonal analysis
               2. Materials
               3. Methods
              4. Concluding remarks
              Acknowledgments
              References
CHAPTER 8 Immunofluorescence analysis of myogenic differentiation
               1. Introduction
               2. Materials
               3. Methods
               4. Immunofluorescence staining of the cells
               5. Notes
               Acknowledgments
               References  :
CHAPTER 9 Generating pancreatic beta-like cells from human pluripotent stem cells
               1. Introduction
               2. Culture and differentiation of hPSCs
              3. Transplantation into kidney capsule of NOD/SCID mice for in vivo maturation
              4. Glucose-stimulated insulin secretion (GSIS) assay
              5. Concluding remarks
              6. Notes
              Acknowledgements
              Declaration of interests
              References
CHAPTER 10 In vitro preparation of human Dental Pulp Stem Cell grafts with biodegradable polymer scaffolds for nerve tissue engineering
              1. Introduction
              2. Materials
              3. Methods
              4. Notes
              5. Concluding remarks
              Acknowledgments
              Disclosures
              References
CHAPTER 11 Identification of progenitor cells and their progenies in adult Drosophila midgut
              1. Introduction
              2. Materials
              3. Methods
              4. Assessment of proliferation and differentiation capability of ISCs
              5. Concluding remarks
              6. Notes
              Acknowledgment
              References
CHAPTER 12 Generation and characterization of human mesenchymal stem/stromal cells for cell therapy applications
               1. Introduction
               2. General considerations
               3. Isolation of MSCs from bone marrow
              4. Isolation of MSCs from human umbilical cord
               5. Cryopreservation and thawing MSCs
               6. Human MSCs characterization
               7. Expected results
              References
CHAPTER 13 Generation of sex-reversed female clonal mice via CRISPR/Cas9-mediated Y chromosome deletion in male embryonic stem cells
               1. Introduction
               2. Materials
               3. Methods
               4. Notes
               5. Concluding remarks
               Acknowledgments
               Disclosures
               References