Plant pathology : method and protocols / edited by Nicola Luchi -- New York, NY : Humana Press, 2022. – (58.17/M592/v.2536)

Contents

Dedication
Preface
Acknowledgments
Contributors
PART I  PLANT DISEASE DIAGNOSIS: TRADITIONAL METHODS
1  Obtaining and Maintaining Cultures of Pinewood Nematodes Bursaphelenchus xylophilus from Wild Dauers
2  Survey and Monitoring of Phytophthora Species in Natural Ecosystems: Methods for Sampling, Isolation, Purification, Storage, and Pathogenicity Tests
3  Field and Laboratory Procedures for Fusarium circinatum Identification and Diagnosis
PART II  DNA EXTRACTION PROTOCOLS
4  Rapid Extraction of Plant Nucleic Acids by Microneedle Patch for In-Field Detection of Plant Pathogens
5  DNA Extraction Methods to Obtain High DNA Quality from Different Plant Tissues
6  A Rapid Method for Extracting High-Quality DNA from Soils
PART III  MOLECULAR METHODS TO DETECT PLANT PATHOGENS
7  Invasive Alien Plant Pathogens: The Need of New Detection Methods
8  Detection of Airborne Inoculum of Hymenoscyphus fraxineus: The Causal Agent of Ash Dieback
9  Molecular Detection of Wheat Blast Pathogen in Seeds
10  Detection and Identification of the Causal Agents of Dothistroma Needle Blight
11  The Use of qPCR to Detect Cryphonectria parasitica in Plants
12  Rapid Molecular Diagnostics in the Field and Laboratory to Detect Plant Pathogen DNA in Potential Insect Vectors
13  A Panel of Keal-Time PCR Assays for the Direct Detection of All of the Xylella fastidiosa Subspecies
14  Selective Quantification of Erwinia amylovora Live Cells in Pome Fruit Tree Cankers by Viability Digital PCR
15  Innovative Detection of the Quarantine Plant Pathogen Curtobacterium flaccumfaciens pv. flaccumfaciens, Causal Agent of Bacterial Wilt of Leguminous Plants
16  Specific Detection of Pseudomonas savastanoi Pathovars: From End-Point PCR to Real-Time PCR and HRMA
PART IV  HIGH-THROUGHPUT SEQUENCING
17  Computational Analysis of HTS Data and Its Application in Plant Pathology
18  Biomonitoring of Fungal and Oomycete Plant Pathogens by Using Metabarcoding
19  Phytobiome Metabarcoding: A Tool to Help Identify Prokaryotic and Eukaryotic Causal Agents of Undiagnosed Tree Diseases
PART V  PLANT-PATHOGEN INTERACTIONS
20  Plant-Fungal Interactions: Laser Microdissection as a Tool to Study Cell Specificity
21  Somatic Embryogenesis as a Tool for Studying Grapevine-Virus Interaction
22  A Quantitative Luminol-Based Assay for KOS Burst Detection in Potato Leaves in Response to Biotic Stimuli
PART VI  POPULATION GENOMICS IN PLANT PATHOLOGY
23  Genetic Analysis of Plant Pathogens Natural Populations
24  Microsatellite Genotyping in the Chestnut Blight Fungus Cryphonectria parasitica
25  A Multiplex PCR Approach to Determine Vegetative Incompatibility Genotypes and Mating Type in Cryphonectria parasitica
PART VII  BIOCONTROL
26  A Detached Leaf Assay for Rapidly Screening Plant Pathogen-Biological Control Agents
27  Spray-Induced Gene Silencing to Study Gene Function in Phytophthora.
28  Plant Sampling for Production of Essential Oil and Evaluation of Its Antimicrobial Activity In Vitro
Index