Yeast protocols / edited by Wei Xiao. -- 4th ed. -- New York, NY : Humana Press, 2021. – (58.17 /M592 /v.2196) |
Contents
Preface
Contributors
1 Use of
Yeast Plasmids: Transformation and Inheritance Assays
2
Construction of Tight Conditional Mutants Using the Improved Auxin-Inducible
Degron (iAID) Method in the Budding Yeast Saccharomyces cerevisiae
3 CRISPR
Nickase-Mediated Base Editing in Yeast
4
Genomic Promoter Shuffling by Using Recyclable Cassettes
5 Study
Essential Gene Functions by Plasmid Shuffling
6 Scarless
Genomic Protein Labeling in Saccharomyces cerevisiae
7
High-Copy Yeast Library Construction and High-Copy Rescue Genetic Screen in
Saccharomyces cerevisiae
8
Genome-Wide Imaging-Based Phenomic Screening Using Yeast (Saccharomyces
cerevisiae) Strain Collections
9
Applications of, Oxford Nanopore Sequencing in Schizosaccharomyces pombe
10
Measuring Genome-Wide Nascent Nucleosome Assembly Using ReIN-Map
11
Targeted Integration of Complex Genetic Elements at Multi-Copy Loci by Golden
Gate Assembly
12 Chromosomal
Rearrangements of Synthetic Yeast by SCRaMbLE
13 Use
Yeast Fab to Construct Genetic Parts and Multicomponent Pathways for Metabolic
Engineering
14 A
Versatile Protocol to Generate Translocations in Yeast Genomes Using
CRISPR/Cas9
15 Yeast
Nucleoplasmic Extracts and an Application to Visualize Chromatin Assembly on
Single Molecules of DNA
16
Assays for Autophagy III: Observing Dynamic Protein Trafficking
17
Isolation of Aged Yeast Cells Using Biotin-Streptavidin Affinity Purification
18
High-Throughput Rapid Yeast Chronological Lifespan Assay
19
Long-Term Imaging and Dynamic Analysis of Cytoophidia in Yeast
20
Monitoring 5'-End Resection at Site-Specific Double-Strand Breaks by Southern
Blot Analysis
Index