DNA vaccines : methods and protocols / edited by Ângela Sousa. -- New York : Humana Press, 2021. – (58.17 /M592 /v.2197)

Contents

PART I  VECTOR DESIGN FOR VACCINATION

1  A Novel Cre Recombinase-Mediated In Vivo Minicircle (CRIM) DNA Vaccine Platform for Veterinary Application

2  Vaccination with Messenger RNA: A Promising Alternative to DNA Vaccination

3  Impact of a Plasmid DNA-Based Alphavirus Vaccine on Immunization Efficiency

PART II  DNA VACCINE ADJUVANTS AND IMMUNOSTIMULATORY RESPONSE

4  CpG Oligonucleotides as Vaccine Adjuvants

5  Molecular Adjuvants for DNA Vaccines: Application, Design, Preparation, and Formulation

6  Assessing Antigen-Specific Cellular Immune Responses upon HIV/SIV Plasmid DNA Vaccination in the Nonhuman Primate Model

PART III  BIOTECHNOLOGICAL PROCESSES TO OBTAIN DNA VACCINES

7  Enhanced Biosynthesis of Plasmid DNA from Escherichia cell Applying Experimental Design

8  Primary Purification of Plasmid DNA Using Differential Isopropanol Precipitation

9  Scale-Up of Plasmid DNA Downstream Process Based on Chromatographic Monoliths

10  Purification of Plasmid DNA by Multimodal Chromatography

11  Minicircle DNA Vaccine Purification and E7 Antigen Expression Assessment

PART IV  DNA VACCINE DELIVERY

12  Tumor-Specific CD8+ T-Cell Responses Induced by DNA Vaccination.

13  Therapeutic DNA Vaccine Against HPV16-Associated Cancer

14  Bulk and Microfluidic Synthesis of Stealth and Cationic Liposomes for Gene Delivery Applications

15  Conception of Plasmid DNA and Polyethylenimine Delivery Systems with Potential Application in DNA Vaccines Field

16  Main Features of DNA-Based Vectors for Use in Lactic Acid Bacteria and Update Protocols

PART V  DNA VACCINE TRANSFER TO CLINICAL TRIALS

17  Ethics of DNA Vaccine Transfer for Clinical Research

18  Preparation of an Academic Clinical Trial

Index