Methods in microbiology. v. 47, Immunological methods in microbiology / edited by Charles S. Pavia, Volker Gurtler. -- London : Elsevier, c2020. – (58.605 /N856 /v.47)

Contents

Contributors

Preface

CHAPTER 1 Introduction to immunological techniques in the clinical laboratory

1. Introduction

2. Basics of the immune response

3. Hypersensitivities

4. Survey of current immunological techniques

5. Conclusion

References

CHAPTER 2 Combining antigen detection and serology for the diagnosis of selected infectious diseases

1. Introduction and historical background

2. Overview of immunoassay development

3. Streptococcus pyogenes (Group A Strept)

4. Streptococcus pneumoniae

5. Legionella pneumophila

6. Helicobacter pylori

7. Coccidioides immitis

8. Cryptococcus neoformans

9. Summary

Financial disclosures and conflicts of interest

References

Further reading

CHAPTER 3 Immunologic detection of Lyme disease and the related borrelioses

1. Introduction

2. Microbiologic features of the Borrelia bacteria

3. Lyme disease

4. Relapsing fever borreliosis

5. Southern tick-associated rash illness

Acknowledgements

Financial disclosures and conflicts of interest

References

Further reading

CHAPTER 4 Immunodetection of bacteria causing brucellosis.

1. Introduction

2. The genus Brucella

3. Epidemiology of Brucella

4. Disease in humans

5. Disease in animals

6. Diagnostic dilemma in brucellosis

7. Immune response to Brucella

8. Antigens of Brucella employed in immunodiagnosis

9. Immunodetection methods for brucellosis

10. Recent developments in brucellosis research

Conclusions and perspective

References

Further reading

CHAPTER 5 Immunological and molecular techniques used for determination of serotypes in Pasteurellaceae

1. Introduction

2. Serotyping

3. Discussion and conclusion

Acknowledgements

References

Further reading

CHAPTER 6 Immunological tests for diarrhoea caused by diarrhoeagenic Escherichia coli targeting their main virulence factors

1. Definition

2. Polyclonal antibodies generation

3. Monoclonal antibodies generation

4. Recombinant antibody generation  .

5. Immunological assays

6. Summary

References

Further reading

CHAPTER 7 Development and improvement of a colony blot immunoassay for the detection of thermotolerant Campylobacter species

1. Introduction

2. Development and improvement of a CBI for Campylobacter spp.: Methods

3. Results and discussion

4. Colony blot immunoassay protocol

Acknowledgements

References

CHAPTER 8 Serodiagnosis of Strongyloides stercoralis infection

1. Strongyloides stercoralis

2. Strongyloidiasis

3. Laboratory diagnosis

4. Serological assays

5. Challenges and way forward

Acknowledgements

References

Further reading

CHAPTER 9 Immunological detection of Zika virus: A summary in the context of general viral diagnostics

1. Introduction

2. Zika virus (ZIKV)

3. Understanding cross-reactivity

4. General diagnostics

5. Immunological diagnostics

6. Serological methods

7. Conclusion

References

CHAPTER 10 Detection of microorganisms using recombinase polymerase amplification with lateral flow dipsticks

1. Introduction

2. Rational of RPA and LEA

3. Materials, equipment and reagents

4. Protocols for RPA-LEA

5. Solutions to potential problems

6. Conclusion and outlook of RPA

Acknowledgements

References

Further reading

CHAPTER 11 Detection of microorganisms with lateral flow test strips

1. Introduction

2. Comparison of bacterial detection methods

3. Principles of LFIA

4, Recognition elements for stability

5. Detector for quantitative, uniform analysis

6. Sensitivity improvement

7. Multi-step analysis

8. Multiplex analysis

9. Cross-reactive Abs and specificity

10. Label-free or immobilised-free format

11. Application of LFIA

12. Future prospects

13. Conclusions

Acknowledgement

References