Stem cells and tissue repair : methods and protocols / edited by Chrissa Kioussi. -- 2nd ed. -- New York, NY : Humana Press, 2020. – (58.17 /M592 /v.2155)

Contents

Contributors

1  Culturing and Manipulating Mouse Embryonic Stem Cells

2  Generation of an Induced Pluripotent Stem Cell Line with the Constitutive EGFP Reporter

3  Engraftable Induced Pluripotent Stem Cell-Derived Neural Precursors for Brain Repair

4  In Vitro Direct Reprogramming of Mouse and Human Astrocytes to Induced Neurons

5  Preparation of Bioscaffolds Delivering Stem Cells for Neural Regeneration

6  Improved Isolation of Human Vascular Wall-Resident Mesenchymal Stem Cells

7  In Vitro Generation of Vascular Wall-Typical Mesenchymal Stem Cells (VW-MSC) from Murine Induced Pluripotent Stem Cells Through VW-MSC-Specific Gene Transfer

8  Analysis of Tooth Innervation in Microfluidic Coculture Devices

9  Peripheral Nerve Regeneration in a Novel Rat Model of Dysphagia

10  Healing of Full-Thickness Murine Skin Wounds Containing Nanofibers Using Splints for Efficient Reepithelialization and to Avoid Contracture

11  Enrichment and Characterization of Human and Murine Pulmonary Mesenchymal Progenitor Cells (MPC)

12  Isolation and Culture of Quiescent Skeletal Muscle Satellite Cells

13  Isolation, Culture, Cryopreservation, and Identification of

14  Long-Term Ex Vivo Expansion of Murine Spermatogonial Stem Cells in a Simple Serum-Free Medium

15  Generating Kidney Organoids from Human Pluripotent Stem Cells Using Defined Conditions

16  Painting the Pancreas in Three Dimensions: Whole-Mount Immunofluorescence Method

17  Induction of Osteoblasts by Direct Reprogramming of Mouse Fibroblasts

Index