Expressed protein ligation : methods and protocols / edited by Miquel Vila-Perello. -- New York : Humana Press, c2020. – (58.17/M592/v.2133) |
Contents
1 Methods and Applications of Expressed Protein
Ligation
2 Protein Splicing: From the Foundations to the
Development of Biotechnological Applications
3 Identification, Characterization, and
Optimization of Split Inteins
4 Methods to Study the Structure and Catalytic
Activity of cis-Splicing Inteins
5 Expressed Protein Ligation: General
Experimental Protocols
6 Peptide Hydrazides as Thioester Equivalents
for the Chemical Synthesis of Proteins
7 Native Chemical Ligation via N-Acylurea
Thioester Surrogates Obtained by Fmoc Solid-Phase Peptide Synthesis
8 Fused Split Inteins: Tools for Introducing
Multiple Protein Modifications
9 Streamlined Expressed Protein Ligation:
Site-Specific Antibody-Drug Conjugate
10 In Vivo Histone Labeling Using Ultrafast
trans-Splicing Inteins
11 Site-Directed Modification of Yeast-Produced
Proteins Using Expressed Protein Ligation
12 Site-Specific Phosphorylation of PDZ Domains
13 Semisynthesis and Reconstitution of
Nucleosomes Carrying Asymmetric Histone Modifications
14 Facile Semisynthesis of Ubiquitylated
Peptides with the Ligation Auxiliary 2 -Aminooxyethanethiol
15 Investigating the Effects of O-GlcNAc
Modifications in Parkinson's Disease Using Semisynthetic α-Synuclein
16 Recombinant Expression of Cyclotides Using
Expressed Protein Ligation
17 Highly Precise Protein Semisynthesis through
Ligation-Desulfurization Chemistry in Combination with Phenacyl Protection of
Native Cysteines.
Index