Myogenesis : methods and protocols / edited by Sissel Beate Ronning. -- New York : Humana Press, c2019. – (58.17/M592/v.1889)

Contents

Preface

Contributors

1  Purification of Myogenic Progenitors from Human Muscle Using Fluorescence-Activated Cell Sorting (FACS)

2  Electrical Pulse Stimulation of Primary Human Skeletal Muscle Cells

3  Transdifferentiation of Muscle Satellite Cells to Adipose Cells Using CRISPR/Cas9-Mediated Targeting of MyoD

4  Chromatin Immunoprecipitation in Skeletal Myoblasts

5  Exercising Bioengineered Skeletal Muscle In Vitro: Biopsy to Bioreactor

6  Isolation and Purification of Satellite Cells from Young Rats by Percoll Density Gradient Centrifugation

7  Lentivirus-Mediated RNAi in Skeletal Myogenesis

8  Adipogenesis from Bovine Precursors

9  Transcriptomic Profiling During Myogenesis

10  Coculture Method to Obtain Endothelial Networks Within Human Tissue-Engineered Skeletal Muscle

11  Interaction Between Skeletal Muscle Cells and Extracellular Matrix Proteins Using a Serum Free Culture System

12  LC-MS Analyses of Lipid Species in Skeletal Muscle Cells and Tissue

13  A siRNA Mediated Screen During C2C12 Myogenesis

14  Fluorescence-Activated Cell Sorting of Larval Zebrafish Muscle Stem/Progenitor Cells Following Skeletal Muscle Injury

15  Preparation of Proliferated Bovine Primary Skeletal Muscle Cells for Bottom-Up Proteomics by LC-MSMS Analysis

16  Myogenesis in Drosophila melanogaster. Dissection of Distinct Muscle Types for Molecular Analysis

17  Measuring Both Glucose Uptake and Myosin Heavy Chain Isoform Expression in Single Rat Skeletal Muscle Fibers

18  Myoblast Phosphoproteomics as a Tool to Investigate Global Signaling Events During Myogenesis

19  Preparation and Culturing of Atlantic Salmon Muscle Cells for In Vitro Studies

20  RNA Interference Screening for Genes Regulating Drosophila Muscle Morphogenesis

Index