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Cell reprogramming for immunotherapy : methods and protocols / edited by Samuel G. Katz and Peter M. Rabinovich. -- New York : Humana Press, c2020. – (58.17/M592/v.2097) |
Contents
Preface
Contributors
PART I
REPROGRAMMING T AND NK CELLS
1 T Cell Reprogramming Against Cancer
2
Identification of Cell Surface Targets for CAR T Cell Immunotherapy
3
Microfluidic Approach for Highly Efficient Viral Transduction
4 Single-Cell Cytokine Analysis to
Characterize CAR-T Cell Activation
5
Testing the Specificity of Compounds Designed to Inhibit CPT1A in T
Cells
6
Engineering of Natural Killer Cells for Clinical Application
7
Dextran Enhances the Lentiviral Transduction Efficiency of Murine and
Human Primary NK Cells
8 In
Vivo Assessment of NK Cell-Mediated Cytotoxicity by Adoptively Transferred
Splenocyte Rejection
9
Immunomodulation of NK Cell Activity
PART II
REPROGRAMMING DIVERSE IMMUNOCYTES
10 An Overview of Advances in Cell-Based
Cancer Immunotherapies Based on the Multiple Immune-Cancer Cell Interactions
11 Rapid Production of Physiologic Dendritic
Cells (phDC) for Immunotherapy
12
Reprogramming Exosomes for Immunotherapy
13
Nanoparticles for Immune Cell Reprogramming and Reengineering of Tumor
Microenvironment
14 CRISPR/Cas9 Gene Targeting in Primary
Mouse Bone Marrow-Derived Macrophages
15
Genome-Wide CRISPRi/a Screening in an In Vitro Coculture Assay of Human
Immune Cells with Tumor Cells
16 The Propagation and Quantification of Two
Emerging Oncolytic Viruses: Vesicular Stomatitis (VSV) and Zika (ZIKV)
PART III
MONITORING REPROGRAMMED IMMUNE CELLS IN VIVO
17 Radiolabeling and Imaging of Adoptively
Transferred Immune Cells by Positron Emission Tomography
18 Functional Analysis of Human
Hematopoietic Stem Cells In Vivo in Humanized Mice
PART IV CLINICAL DESIGN FOR CELL THERAPY
19 Monitoring Allogeneic CAR-T Cells Using
Flow Cytometry
20 Key Factors in Clinical Protocols for
Adoptive Cell Therapy in Melanoma
21
Place of Academic GMP Facilities in Modern Cell Therapy
Index
