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Mait cells : methods and protocols / edited by Helen Kaipe, Isabelle Magalhaes. -- New York : Humana Press, c2020. – (58.17/M592/v.2098) |
Contents
Preface
Contributors
PART I
MAIT CELLS IN HUMAN TISSUES AND BLOOD
1
MAIT Cells in Health and Disease
2
Isolation and Characterization of MAIT Cells from Human Tissue Biopsies
3
Isolation and Characterization of MAIT Cells from Tumor Tissues
4
Isolation of Immune Cells from Placental Tissues and Phenotypic and
Functional Analysis of MAIT Cells
5
Methods for High-Dimensional Flow Cytometry Analysis of Human MAIT Cells
in Tissues and Peripheral Blood
6 In
Situ Detection of MAIT Cells and MR1-Expressing Cells in Tissue Biopsies
Utilizing Immunohistochemistry
PART II
FUNCTIONAL ANALYSIS OF MAIT CELLS
7
Human MAIT Cell Activation In Vitro
8
Interactions Between MAIT Cells and Dendritic Cells
9
Influenza A Virus-Infected Lung Epithelial Cell Co-Culture with Human
Peripheral Blood Mononuclear Cells
10
Quantification of Human MAIT Cell-Mediated Cellular Cytotoxicity and
Antimicrobial Activity
11
MAIT Cell Activation by Fungal Pathogens
12 A
Flow Chamber Assay for Studying MAIT Cell Trafficking
PART III
MONITORING AND IN VITRO AMPLIFICATION Or MAIT CELLS
13
Production of MR1 Tetramers Loaded with Microbial Ligands
14
Immune Monitoring of Human Mucosal-Associated Invariant T Cells by
Quantitative Proteomics
15
Generation of MR1-Restricted T Cell Clones by Limiting Dilution Cloning
of MR1 Tetramer+ Cells
16
Reprogramming of MAIT Cells to Pluripotency and Redifferentiation
PART IV MAIT CELLS IN MU~ MODELS
17
Study of MAIT Cell Activation in Viral Infections In Vivo
18
MAIT Cells in Type 1 Diabetes Mouse Models
19
Magnetic Bead-Based Enrichment of Murine MAIT Cells
Index
