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Circulating nucleic acids in serum and plasma : CNAPS IX / edited by Peter B. Gahan, Michael Fleischhacker, Bernd Schmidt. -- Switzerland : Springer International Publishing, 2016. – (58.17425083/C578)

Contents

Part I  Nucleic Acids in Oncology - Prognosis, Treatments Screening and Metastases

1   Circulating Cell-Free miR-373, miR-200a, miR-200b and miR-200c in Patients with Epithelial Ovarian Cancer

2   Cell-Free miRNA-141 and miRNA-205 as Prostate Cancer Biomarkers

3   Clinical Utility of Circulating Tumor DNA for Molecular Assessment and Precision Medicine in Pancreatic Cancer

4   An Enquiry Concerning the Characteristics of Cell-Free DNA Released by Cultured Cancer Cells

5   Detection of p53 Mutations in Circulating DNA of Transplanted Hepatocellular Carcinoma Patients as a Biomarker of Tumor Recurrence

6   Unbiased Detection of Somatic Copy Number Aberrations in cfDNA of Lung Cancer Cases and High-Risk Controls with Low Coverage Whole Genome Sequencing

7   Liquid Profiling in Lung Cancer - Quantification of Extracellular miRNAs in Bronchial Lavage

8   Screening of KRAS Mutation in Pre- and Post-Surgery Serum of Patients Suffering from Colon Cancer by COLD-PCR HRM

9   Non-dividing Cell Virtosomes Affect In Vitro and In Vivo Turnout Call Replication

10  Features of Circulating DNA Fragmentation in Blood of Healthy Females and Breast Cancer Patients

11  Liquid Profiling of Circulating Nucleic Acids as a Novel Tool for the Management of Cancer Patients

Part II  CNAPS in Foetal Medicine

12  Characterization of Human Pregnancy Specific Glycoprotein (PSG) Gene Copy Number Variations in Pre-eclampsia Patients

13  Non-invasive Prenatal Diagnosis of Feto-Maternal Platelet Incompatibility by Cold High Resolution Melting Analysis

14  Implementing Non-Invasive Prenatal Diagnosis (NIPD) in a National Health Service Laboratory; From Dominant to Recessive Disorders

Part III  Biology of CNAPS

15  Comparative Analysis of Harmful Physical Factors Effect on the Cell Genome

16  Heterochromatic Tandem Repeats in the Extracellular DNA

17  A Historical and Evolutionary Perspective on Circulating Nucleic Acids and Extracellular Vesicles: Circulating Nucleic Acids as Homeostatic Genetic Entities

18  Comparison of MicroRNA Content in Plasma and Urine Indicates the Existence of a Transrenal Passage of Selected MicroRNAs

19  A Quantitative Assessment of Cell-Free DNA Utilizing Several Housekeeping Genes: Measurements from Four Different Cell Lines

20  Oligodeoxynucleotide Analogues of Circulating DNA Inhibit dsRNA-Induced Immune Response at the Early Stages of Signal Transduction Cascade in a Cell Type-Dependent Manner

21  GC-Rich DNA Fragments and Oxidized Cell-Free DNA Have Different Effects on NF-kB and NRF2 Signaling in MSC

22  Evaluation of the State of Transplanted Liver Health by Monitoring of Organ-Specific Genomic Marker in Circulating DNA from Receptor

23  Vesicular and Extra-Vesicular RNAs of Human Blood Plasma

24  Artificial Analogues of Circulating Box C/D RNAs Induce Strong Innate Immune Response and MicroRNA Activation in Human Adenocarcinoma Cells

25  Multiple Ways of cfDNA Reception and Following ROS Production in Endothelial Cells

26  Protein Content of Circulating Nucleoprotein Complexes

Part IV  Methodology

27  Digital PCR of Genomic Rearrangements for Monitoring Circulating Tumour DNA

28  mFast-SeqS as a Monitoring and Pre-screening Tool for Tumor-Specific Aneuploidy in Plasma DNA

29  Methodological Variables in the Analysis of Cell-Free DNA

30  Novel Technology for Enrichment of Biomolecules from Cell-Free Body Fluids and Subsequent DNA Sizing

31  A Rapid and Sensitive Method for Detection of the T790M Mutation of EGFR in Plasma DNA

32  Evaluation of Different Blood Collection Tubes and Blood Storage Conditions for the Preservation and Stability of Cell-Free Circulating DNA for the Analysis of the Methylated mSEPT9 Colorectal Cancer Screening Marker

33  Purification of Circulating Cell-Free DNA from Plasma and Urine Using the Automated Large-Volume Extraction on the QIAsymphony® SP Instrument

34  Detection and Quantification of KIT Mutations in ctDNA by Plasma Safe-SeqS

Part V  Ethics

35  Lost in Translation? Ethical Challenges of Implementing a New Diagnostic Procedure

Part VI  Round-Table

36  Academia Meets Industry

Index