Microbial biopolyester production, performance and processing : microbiology. feedstocks, and metabolism / edited by Martin Koller. -- Sharjah : Bentham Science Publishers, 2016. – (58.696/M626a)

Contents

FOREWORD    i

PREFACE  iv

     THE CONTRIBUTING SCIENTISTS  v

     CHAPTER 1  vii

     CHAPTER 2  viii

     CHAPTER 3  viii

     CHAPTER 4  ix

     CHAPTER 5  ix

     CHAPTER 6  x

     CHAPTER 7  xi

     CHAPTER 8  xi

LIST OF CONTRIBUTORS    xiv

CHAPTER 1  NOVEL INEXPENSIVE FEEDSTOCKS FROM AGRICULTURE AND INDUSTRY FOR MICROBIAL POLYESTER PRODUCTION  3

     GENERAL ASPECTS OF POLYHYDROXYALKANOATE PRODUCTION  3

     PRODUCTION OF PHA USING LIPID SUBSTRATES  13

     MOLASSES  43

     CHEESE WHEY  48

     GLYCEROL AS A CHEAP SUBSTRATE FOR PHA PRODUCTION  58

     STARCH-BASED SUBSTRATES  64

     STARCHY MATERIALS    66

     PETROCHEMICAL PLASTIC WASTE TRANSFORMATION TO PHA  74

     PRODUCTION OF PHA FROM VOLATILE FATTY ACIDS  77

     CONCLUDING REMARKS  78

     CONFLICT OF INTEREST  79

     ACKNOWLEDGEMENTS  79

     REFERENCES  80

CHAPTER 2  PHA BIOPOLYESTER PRODUCTION FROM SURPLUS WHEY: MICROBIOLOGICAL AND ENGINEERING ASPECTS  100

     THE NEED FOR VALUE-ADDED AND SUSTAINABLE UPGRADING OF WHEY: HOW TO TURN A SURPLUS PRODUCT INTO A USEFUL RAW MATERIAL  101

     WHAT CHARACTERIZES PHASS AS SO CALLED "GREEN PLASTICS"?  107

     THE ENTIRE PROCESS TO CONVERT THE RAW MATERIAL (MILK) TO PHA BIOPOLYMERS  131

     CONCLUDING REMARKS    153

     CONFLICT OF INTEREST  155

     ACKNOWLEDGEMENTS  155

     REFERENCES  156

CHAPTER 3  BIOPOLYESTERS AND RELATED VALUABLE PRODUCTS IN PHOTOTROPHIC MICROBES  175

     INTRODUCTION  176

     CONCLUDING REMARKS  208

     CONFLICT OF INTEREST  208

     ACKNOWLEDGEMENTS  209

     NOTES    209

     REFERENCES  209

CHAPTER 4  BIOPOLYMER PRODUCTION  BY  MIXED  MICROBIAL  CULTURES: INTEGRATING REMEDIATION WITH VALORIZATION  226

     INTRODUCTION  227

     APPLICATIONS  254

     CONCLUSION  256

     CONFLICT OF INTEREST  256

     ACKNOWLEDGEMENTS  256

     REFERENCES  256

CHAPTER 5  RALSTONIA EUTROPHA AND THE  PRODUCTION  OF VALUE ADDED PRODUCTS: METABOLIC BACKGROUND OF THE WILD-TYPE STRAIN AND ITS ROLE AS A DIVERSE, GENETICALLY-ENGINEERED BIOCATALYST ORGANISM  265

     INTRODUCTION  266

    POLYHDROXYALKANOATE HOMEOSTASIS  270

     THE BUSINESS OF PHA:  R. EUTROPHA  AS  A  PLATFORM  ORGANISM  FOR INDUSTRIAL CULTIVATIONS  284

    MORE THAN JUST A GOOD IDEA: UTILITY OF PHA BIOPOLYMERS  298

     CONCLUDING REMARKS: RALSTONIA WRAP UP    324

    CONFLICT OF INTEREST  324

     ACKNOWLEDGEMENTS  324

     NOTES  325

     REFERENCES    325

CHAPTER 6  PHBHV BIOSYNTHESIS BY HALOFERAX MEDITERRANEI: FROM GENETICS, METABOLISM, AND ENGINEERING TO ECONOMICAL PRODUCTION  348

     INTRODUCTION  349

     THE GENOME OF H. MEDITERRANEI  350

     THE KEY ENZYMES FOR PHBHV BIOSYNTHESIS  352

     MULTIPLE PATHWAYS FOR PROPIONYL-COA SUPPLYING  360

     ENZYMES AND PATHWAYS FOR PHBHV MOBILIZATION    362

     PRODUCTION OF PHBHV FROM INEXPENSIVE CARBON RESOURCES  366

     ENGINEERING AND TAILOR-MADE PRODUCTION OF PHBHV  369

     CONCLUDING REMARKS  373

     NOTES  374

     CONFLICT OF INTEREST  374

     ACKNOWLEDGEMENTS  374

     REFERENCES  374

CHAPTER 7  BACTERIAL GENETIC MODIFICATIONS  FOR  IMPROVING POLYHYDROXYALKANOATES PRODUCTION FROM INEXPENSIVE CARBON SOURCES    380

     TRADITIONAL AND DNA-BASED METHODS TO LOOK FOR PHA PRODUCERS  381

     MAKING PHA PRODUCING STRAINS ABLE TO UTILIZE DIFFERENT CARBON SOURCES   389

     CLONING PHA GENES IN NON PRODUCING BACTERIA   406

     CONCLUDING REMARKS  414

     CONFLICT OF INTEREST  416

     ACKNOWLEDGEMENTS  416

     REFERENCES  417

 CHAPTER 8  MEDIUM-CHAIN-LENGTH POLY-3-HYDROXYALKANOATES  436

     INTRODUCTION  436

     DISCOVERY AND POTENTIAL APPLICATIONS  437

     METABOLIC ROUTES TO MCL-PHA  439

     NATURALLY OCCURRING MCL-PHA PRODUCING MICROORGANISMS  445

     MCL-PHA MATERIAL PROPERTIES  447

     PRODUCTION METHODS AND ECONOMIC CONSIDERATIONS  450

     SEPARATION METHODS  456

    THE FUTURE  462

     CONFLICT OF INTEREST  463

     ACKNOWLEDGEMENTS  463

     REFERENCES  464

SUBJECT INDEX  474