Preservation of cells : a practical manual / by Allison Hubel. -- Hoboken, NJ : John Wiley & Sons, c2018. – (58.15073/H877)

Contents

Preface  xiii

Acknowledgments  xvii

Nomenclature  xix

1  Introduction  1

2  Pre-freeze Processing and Characterization  15

3  Formulation and Introduction of Cryopreservation Solutions  29

4  Freezing Protocols  47

5  Storage and Shipping of Frozen Cells  65

6  Thawing and Post-Thaw Processing  85

7  Post-Thaw Assessment  97

8  Algorithm-Driven Protocol Optimization  111

PROTOCOLS

Introduction  117

Cryopreservation of Endothelial Cells in Suspension  119

Cryopreservation of Peripheral Blood Mononuclear Cells from Whole Blood  125

Cryopreservation of Human Adipose Stem Cells  131

Cryopreservation of Red Blood Cells  141

Cryopreservation of Oocytes by Slow Freezing  147

Oocyte Vitrification and Warming  155

Transportation of Hematopoietic Progenitor Cells and Other Cellular Products  163

Cryopreservation of Hematopoietic Progenitor Cells  167

Thawing of Hematopoietic Progenitor Cells  175

Processing and Cryopreservation of T-Cells  179

Thawing and Reinfusion of Cryopreserved T-Cells  183

Index  189