Immunohistochemistry and immunocytochemistry : essential methods / edited by Simon Renshaw. -- 2nd ed. -- Chichester, West Sussex, UK : Wiley Blackwell, 2017. – (63.172/I33u/2nd ed.)

Contents

List of Contributors  vii

Preface  ix

Acknowledgements  xi

Chapter 1: Antibodies for Immunochemistry

     Introduction

     Immunogens for Antibody Production

     Antibody Production

     Antibody Purification

     Fragment Antibody Preparations

     Antibody Labelling

     Antibody Stability and Storage

     References

Chapter 2: The Selection of Reporter Labels

     Introduction

     Enzymatic Labels

     Fluorescence Detection

     References

Chapter 3: Immunohistochemistry and Immunocytochemistry

     Specimen Formats for Immunochemistry

     Fixation

     Processing Tissue Blocks to Paraffin Wax

     Microtomy

     Tissue Microarrays

     Specimen Storage

     Decalcification

     Antigen Retrieval

     Controls

     Immunochemical Staining Techniques (Optimizing a New Antibody)

     Counterstains

     Mounting

     Troubleshooting

     Examples of Immunostaining Photomicrographs

     Acknowledgements

     References

Chapter 4: Multiple Immunochemical Staining Techniques

     Introduction

     Methods and Approaches

     References

Chapter 5: Quality Assurance in Immunochemistry

     Introduction

     Methods and Approaches

     Automated Immunochemical Staining

     Troubleshooting

     References

Chapter 6: Automated Immunochemistry

     Introduction

     Methods and Approaches

     Other Forms of Automation

     References

Chapter 7: Confocal Microscopy

     Introduction

     When Should Confocal be Used?

     Applications: For Example Co-localization, Quantification, 3D Visualization and Kinetics

     How To Set Up a Confocal Experiment?

     References

     Further Readings

Chapter 8: Ultrastructural Immunochemistry

     Introduction

     Methods and Approaches

     References

Index