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The fundamentals of scientific research : an introductory laboratory manual / by Marcy A. Kelly ; with contributions from Pryce L. Haddix. -- Hoboken, New Jersey : Wiley Blackwell, c2015. – (58.15073/K29)

Contents

Preface

Acknowledgments

About the Companion Website

Introduction

Module 1 Working with and Learning About Common Laboratory Techniques and Equipment

Exercise 1A  Using Common Laboratory Tools to Evaluate Measurements Pre-laboratory Thinking Questions

Exercise 1B  Using Common Laboratory Tools to Evaluate Measurements

Exercise 1C  Using Common Laboratory Tools to Evaluate Measurements Post-laboratory Thinking Questions

Exercise 2A  Using Microscopy to Evaluate Cell Size and Complexity Pre-laboratory Thinking Questions

Exercise 2B  Using Microscopy to Evaluate Cell Size and Complexity

Exercise 2C  Using Microscopy to Evaluate Cell Size and Complexity Post-laboratory Thinking Questions

Exercise 3A  The Bacterial Growth Curve Pre-laboratory Thinking Questions

Exercise 3B  The Bacterial Growth Curve

Exercise 3C  The Bacterial Growth Curve Post-laboratory Thinking Questions

Module 2 Working with and Learning About Serratia marcescens in the Laboratory

Exercise 4A  Protein Concentration Versus Growth Stage Pre-laboratory Thinking Questions

Exercise 4B  Protein Concentration Versus Growth Stage

Exercise 4C  Protein Concentration Versus Growth Stage Post-laboratory Thinking Questions

Exercise 5A  Measuring Prodigiosin Pre-laboratory Thinking Questions

Exercise 5B  Measuring Prodigiosin

Exercise 5C  Measuring Prodigiosin Post-laboratory Thinking Questions

Exercise 6A  Conditions Affecting the Growth of and Prodigiosin Production by Serratia marcescens Pre-laboratory Thinking Questions

Exercise 6B  Conditions Affecting the Growth of, and Prodigiosin Production by

 S. marcescens

Exercise 6C  Formal Laboratory Report Describing the Conditions Affecting the Growth of and Prodigiosin Production by S. marcescens

Exercise 7A  Biochemistry of Prodigiosin Production Pre-laboratory Thinking Questions

Exercise 7B  Biochemistry of Prodigiosin Production

Exercise 7C  Biochemistry of Prodigiosin Production Post-laboratory Thinking Questions

Exercise 8A  The Probability Basis for Mutation Rate Calculation: A Dice-Roll Exercise Pre-laboratory Thinking Questions

Exercise 8B  The Probability Basis for Mutation Rate Calculation: A Dice-Roll Exercise

Exercise 8C  The Probability Basis for Mutation Rate Calculation: A Dice-Roll Exercise Post-laboratory Thinking Questions

Exercise 9A  Understanding Evolution by the Generation of UV Light-Induced Prodigiosin Mutants Pre-laboratory Thinking Questions

Exercise 9B  Understanding Evolution by the Generation of UV Light-induced Prodigiosin Mutants

Exercise 9C  Understanding Evolution by the Generation of UV Light-induced Prodigiosin Mutants Post-laboratory Thinking Questions

Exercise 10A  Understanding the Energy Spilling Properties of Prodigiosin Pre-laboratory Thinking Questions

Exercise 10B  Understanding the Energy Spilling Properties of Prodigiosin

Exercise 10C  Understanding the Energy Spilling Properties of Prodigiosin Post-laboratory Thinking Questions

Module 3 Initial Characterization of Novel Serratia marcescens Prodigiosin Mutants

Exercise llA  Prodigiosin Mutant Study Part 1 Pre-laboratory Thinking Questions

Exercise llB  Prodigiosin Mutant Study Part 1

Exercise llC  Prodigiosin Mutant Study Part 1 Post-laboratory Thinking Questions

Exercise 12A  Prodigiosin Mutant Study Part 2 Pre-laboratory Thinking Questions

Exercise 12B  Prodigiosin Mutant Study Part 2

Exercise 12C  Formal Laboratory Report 2: Prodigiosin Mutant Study

Appendix A  CSE Citation and Reference List Format Guidelines

Appendix B  Prodigiosin Biosynthesis

References

Index