Advanced biophysics / edited by Lucas Bryant. -- New York : Magnum Publishing, c2017 .—(58.171 /A244)

Contents

Chapter 1   Comparative Analysis of the Informative Value of Radioimmunoassay and Laser Correlation Spectroscopy in Myasthenia Gravis

Abstract

1.  Introduction

2.  Materials' and Methods of the Study

3.  Results and Discussion

4.  Conflict of Interests

References

Chapter 2   Ultrafast Fluorescence Spectroscopy via Upconversion: Applications to Biophysics Mortality after myocardial infarction in patients with diabetes mellitus

Abstract  13

1. Introduction  13

2. Basic Concepts    16

3. Upconversion Spectrophotofluorometer and Experimental Considerations  22

4. Ultrafast Photophysics of Single Tryptophan, Peptides, Proteins, and Nucleic Acids  25

S. Summary and Future Directions  34

References  34

Chapter 3   AIM for Allostery: Using the Ising Model to Understand Information Processing and Transmission in Allosteric Biomolecular Systems

Abstract

1. Introduction

2. Results and Discussion

3. Conclusions

4. Acknowledgments

5. Conflicts of Interest

References

Chapter 4 Direct Determination of Chitosan-Mucin Interactions Using a Single-Molecule Strategy: Comparison to Alginate-Mucin Interactions

Abstract

1. Introduction

2. Experimental Section

3. Results and Discussion

4. Energy Landscape of the Mucin--Chitosan Interactions

5. Conclusions

6. Supplementary Materials

7. Acknowledgments

8. Conflicts of Interest

References

Chapter 5   A Structural Switch between Agonist and Antagonist Bound Conformations for a Ligand-Optimized Model of the Human Aryl Hydrocarbon Receptor Ligand Binding Domain  103

Abstract  103

1.  Introduction  104

2.  Experimental Section  106

3.  Results  112

4.  Molecular Dynamics Simulations  116

5.  Discussion  124

6.  Conclusions  128

7.  Acknowledgments  128

8.  Conflicts of Interest  128

References  128

Chapter 6  Teaching Biophysics. Suggestions For The Introductory Lecture in Biophysics

Abstract

1.  Introduction

2.  Principal Points to be Approached

3.  Biophysics all Around the World

4.  Utility of Biophysicists. are they Needed?

5.  Conclusion

References

Chapter 7   Computational Models of Neuronal Biophysics and the Characterization of Potential Neuropharmacological Targets  143

Abstract

1.  Brain Conditions and Neuronal Drug Targets

2.  Computational Neuroscience.

3.  Drug Databases

4.  Compartmental Simulations of Neuronal Electrophysiology

5.  Signaling Pathway Models

6.  Conclusions

7.  Acknowledgements

References

Chapter 8   Molecular Modelling Study of the PPAR Receptor in Relation to the Mode of Action/Adverse Outcome Pathway Framework for liver Steatosis

Abstract  171

1.  Introduction  172

2.  Results and Discussion  174

3.  Experimental Section  183

4.  Conclusions  185

5.  Supplementary Information  185

6.  Acknowledgments    185

7.  Conflicts of Interest  185

References  185

Chapter 9   Kinetics and Thermodynamics of Membrane Protein Folding  191

Abstract  191

1.  Introduction  191

2.  Basic Strategies for Studying Protein Folding and Stability 

3.  Temperature Induced Membrane Protein Denaturation  196

4.  Solvent Denaturation of Membrane Proteins  198

5.  Conclusions  208

6.  Acknowledgments  209

7.  Conflicts of Interest  209

References  209

Chapter 10   Hypoxia Reduces the Efficiency of Elisidepsin by Inhibiting Hydroxylation and Altering the Structure of Lipid Rafts  219

Abstract  219

1.  Introduction  220

2.  Results  221

3.  Discussion  229

4.  Experimental Section  232

5.  Conclusions  236

6.  Acknowledgments  236

7.  Conflicts of Interest  236

References  236

Chapter 11   Conformational Motions and Functionally Key Residues for Vitamin B12 Transporter BtuCD-BtuF Revealed by Elastic Network Model with a Function-Related Internal Coordinate  241

Abstract  241

1.  Introduction  242

2.  Results and Discussion  244

3.  Experimental Section  254

4.  Conclusions  259

5.  Supplementary Materials  260

6.  Acknowledgments  260

References  260

Chapter 12  Design and Characterization of a Sensorized Microfluidic Cell-Culture System with Electro-Thermal Micro-Pumps and Sensors for Cell Adhesion, Oxygen, and pH on a Glass Chip

Abstract

1.  Introduction

2.  Experimental Section

3.  Results and Discussion

4.  Conclusions

5.  Acknowledgments

6.  Conflicts of Interest

References