Culture of animal cells : a manual of basic technique and specialized applications / R. Ian Freshney. -- Seventh edition. -- Hoboken, New Jersy : Wiley Blackwell, c2016. – (59.105 /F885 /7th ed.)

Contents

List of Figures, xix

List of Color Plates, xxiii

List of Tables, xxv

List of Protocols, xxvii

List of Minireviews, xxix

Preface and Ackniowledgments, xxxi

Abbreviations, xxxiii

About the Companion l/Vebsite xxxviii

1. Introduction, 1

2. Biology of Cultured Cells, 19

3. Laboratory Design and Layout, 37

4. Equipment and Materials, 51

5. Aseptic Technique, 73

6. Safety, Bioethics, and Validation, 89

7. Culture Vessels and Substrates, 111

8 Defined Media and Supplements,

9 Serum-Free Media, 149

10. Preparation and Sterilization, 173

11. Primary Culture, 207

12. Subculture and Cell Lines, 235

13. Authentication and Validation, 259

14. MicrobialContamination, 289

15. Cryopreservation and Banking, 307

16. Cloning and Selection, 327

17. Cell Sorting, 347

18. Cell Line Characterization, 359

19. Differentiation, 383

20. Three-Dimensional Culture, 401

21. Scale-up and Automation, 427

22. Senescence, Immortalization, and Transformation, 455

23. Quantitation, 483

24. Cytotoxicity, 513

25. Culture of Specific Cell Types, 531

26. Stem Cells, 571

27. Training Programs, 591

28. Problem Solving, 627

29. In Conclusion, 651

Supplementary Material