Biophysics : tools and techniques / Mark C. Leake. -- Boca Raton, FL : CRC Press, Taylor & Francis Group, 2016. – (58.171 /L435b)

Contents

Preface

Author

Chapter 1  Introduction:Toolbox at the Physical-Life Science Interface

1.1   Motivation for Biophysics

1.2   What Do We Mean by a "Toolbox?".

1.3   Makeup of the Subsequent Chapters in This Book

1.4   Once More, unto the Breach

Questions

References

Chapter 2  Orientation for the Bio-Curious: The Basics of Biology for the Physical Scientist

2.1   Introduction: The Material Stuff of Life  11

2.2   Architecture of Organisms, Tissues, and Cells and the Bits Between  13

2.3   Chemicals That Make Cells Work  19

2.4   Cell Processes  32

2.5   Physical Quantities in Biology  42

2.6   Summary Points

Questions

References

Chapter 3  Making Light Work in Biology: Basic, Foundational Detection and Imaging Techniques Involving Ultraviolet, Visible, and Infrared Electromagnetic Radiation Interactions with Biological Matter

3.1   Introduction

3.2   Basic UV-VIS-IR Absorption, Emission, and Elastic Light Scattering Methods

3.3   Light Microscopy Basics

3.4   Nonfluorescence Microscopy

3.5   Fluorescence Microscopy: The Basics

3.6   Basic Fluorescence Microscopy Illumination Modes

3.7   Summary Points

Questions

References

Chapter 4  Making Light Work Harder in Biology: Advanced, Frontier UV-VIS-IR Spectroscopy and Microscopy for Detection and Imaging

4.1   Introduction

4.2   Super-Resolution Microscopy

4.3   EOrster Resonance Energy Transfer  111

4.4   Fluorescence Correlation Spectroscopy  116

4.5   Light Microscopy of Deep Samples  119

4.6   Advanced Biophysical Techniques Using Elastic Light Scattering  126

4.7   Tools Using the Inelastic Scattering of Light  131

4.8   Summary Points  134

Questions  134

References  137

Chapter 5  Detection and Imaging Tools That Use Nonoptical Waves: Radio and Microwaves, Gamma and X-Rays, and Various High-Energy Particle Techniques  139

5.1   Introduction  139

5.2   Electron Microscopy  140

5.3   X-Ray Tools

5.4   NMR and Other Radio Frequency and Microwave Resonance Spectroscopies

5.5   Tools That Use Gamma Rays, Radioisotope Decays, and Neutrons

5.6   Summary Points

Questions.

References

Chapter 6  Forces: Methods That Measure and/or Manipulate Biological Forces or Use Forces in Their Principle Mode of Operation on Biological Matter

6.1   Introduction  177

6.2   Rheology and Hydrodynamics Tools  179

6.3   Optical Force Tools  ]83

6.4   Magnetic Force Methods  ]98

6.5   Scanning Probe Microscopy and Force Spectroscopy

6.6   Electrical Force Tools

6.7   Tools to Mechanically Probe Cells and Tissues

6.8   Summary Points  226

Chapter 7  Complementary Experimental Tools: Valuable Experimental Methods That Complement Mainstream Research Biophysics Techniques  233

7.1   Introduction  233

7.2   Bioconjugation  233

7.3   Model Organisms  237

7.4   Molecular Cloning  241

7.5   Making Crystals  248

7.6   High-Throughput Techniques  253

7.7   Characterizing Physical Properties of Biological Samples

7.8   Biomedical Physics Tools

7.9   Surfimary Points

Questions

References

Chapter 8  Theoretical Biophysics: Computational Biophysical Tools, and Methods That Require a Pencil and Paper  277

8.1   Introduction  277

8.2   Molecular Simulation Methods  278

8.3   Mechanics of Biopolymers  302

8.4   Reaction, Diffusion, and Flow  310

8.5   Advanced In Silico Analysis Tools  326

8.6   Rigid-Body and Semirigid-Body Biomechanics  337

8.7   Summary Points  341

Questions  342

References  346

Chapter 9  Emerging Biophysics Techniques: An Outlook of the Future Landscape of Biophysics Tools  347

9.1   Introduction  347

9.2   Systems Biology and Biophysics: "Systems Biophysics".  347

9.3   Synthetic Biology and Bionanotechnology  353

9.4   Personalizing Healthcare  366

9.5   Extending Length and Time Scales to Quantum and Ecological Biophysics  371

9.6   Summary Points  379

Questions  379

References  380

Index  383