 |
Methods in biotechnology / Seung-Beom Hong, M. Bazlur Rashid, Lory Z. Santiago-Vazquez. -- Hoboken, New Jersey : Wiley Blackwell, c2017. – (58.205 /H772) |
Contents
Preface, vii
Acknowledgements, ix
Introduction to biotechnology lab, xi
About the companion website, xix
Part I: METHODS IN BIOTECHNOLOGY (MB)
LABORATORY EXERCISES
1 MB
experiment 1: Lab measurements, 3
2 MB
experiment 2: Use of the spectrophotometer and Beer's law, 9
3 MB
experiment 3: Making solutions and buffer efficacy, 15
4 MB experiment
4: Acid-base titration, 19
5 MB
experiment 5: Protein denaturation and precipitation, 23
6 MB
experiment 6: Bacterial transformation, 27
7 MB
experiment 7: GFP purification, 33
8 MB
experiment 8: SDS-PAGE analysis, 37
9 MB
experiment 9: DNA isolation, 41
10 MB experiment 10: PCR-based Alu.human DNA
typing, 45
11 MB experiment 11: Restriction enzyme
digestion, 51
12 MB experiment 12" Agarose gel
electrophoresis, 53
13 MB experiment 13: Ouchterlony and ELISA
immunoassays, 57
14 MB experiment 14: Testing plant
substances for antimicrobial activity, 63
15 MB experiment 15: Peroxidase enzyme
activity assay, 67
Part II: ADVANCED METHODS IN BIOTECHNOLOGY
(AMB) 1 LABORATORY EXERCISES
16 AMB 1 experiment 16: Aseptic technique
and culture handling, 73
17 AMB 1 experiment 17: Yeast culture media
preparation, 77
18 AMB 1 experiment 18: Growth curve, 79
19 AMB 1 experiment 19: Mini plasmid prep,
83
20 AMB 1 experiment 20: Restriction
digestion, purification, concentration, and quantification of DNA, 87
21 AMB 1 experiment 21: Polymerase chain
reaction (PCR), 91
22 AMB 1 experiment 22: TA, blunt end, SLIC,
and CPEC cloning of PCR product, 97
23 AMB 1 experiment 23: One-step
multifragment assembly cloning, 103
24 AMB 1 experiment 24: Restriction enzyme
digestion and fast agarose gel electrophoresis, 111
25 AMB 1 experiment 25: Southern blot
transfer, 117
26 AMB 1 experiment 26: Probe labeling and
purification, 121
27 AMB 1 experiment 27: Dot blot assay, 125
28 AMB 1 experiment 28: Pre-hybridization,
hybridization, and detection, 127
29 AMB 1 experiment 29: Total yeast RNA
isolation and RT-PCR, 131
30 AMB 1 experiment 30: Yeast-based in vivo
recombination cloning, 139
31 AMB 1 experiment 31: Plasmid DNA
isolation from yeast, 143
32 AMB 1 experiment 32: E. coli transformation
with yeast plasmid DNA, 145
33 AMB 1 experiment 33: X-gal filter lift assay,
147
34 AMB 1 experiment 34: Protein quantitation
assay, 149
35 AMB 1 experiment 35: Quantitative β-Galactosidase assay in
yeast, 155
