Transgenic plants : methods and protocols / edited by Jim M. Dunwell, Andy C. Wetten. — 2nd ed. — New York : Humana Press ; Springer, c2012. – (58.17/M592/v.847)

Contents

    CONTENTS
    
    Preface
    Contributors
    PART I SELECTION AND DETECTION METHODS
    1 Employment of Cytokinin Vectors for Marker-Free and Backbone-Free Transformation
    2 Organophosphorus Hydrolase: A Multifaceted Plant Genetic Marker Which Is Selectable， Scorable， and Quantifiable in Whole Seed
    3 Use of Northern Blotting for Specific Detection of Small RNA Molecules in Transgenic Plants
    PART II TRANSFORMATION TECHNOLOGY: LOWER PLANTS
    4 Genetic Transformation of the Model Green Alga Chlamydomonas reinhardtii 35
    PART III TRANSFORMATION TECHNOLOGY: RICE
    5 A High-Efficiency Agrobacterium-Mediated Transformation System of Rice ( Oryza sativa L.)
    6 Selection of Transgenic Rice Plants Using a Herbicide Tolerant Form of the Acetolactate Synthase Gene
    7 Visual Selection in Rice: A Strategy for the Efficient Identification of Transgenic Calli Accumulating Transgene Products
    8 Characterization of Rice Genes Using a Heterologous Full-Length eDNA Expression System
    9 Bioactive Bead-Mediated Transformation of Plants with Large DNA Fragments
    PART IV TRANSFORMATION TECHNOLOGY: CEREALS AND OTHER MONOCOTS
    10 Agrobacterium-Mediated Transfbrmation of Sorghum bicolor Using Immature Embryos
    11 Split-Transgene Expression in Wheat
    12 Agrobacterium-Mediated Transformation of Brachypodium distachyon
    13 Transformation of Barley (Hordeum vulgare L.) by Agrobacterium tumefaciens
    14 Biolistic-Mediated Production of Transgenic Oil Palm 163
    15 Transformation of Oil Palm Using Agrobacterium tumefaciens 177
    PART V TRANSFORMATION TECHNOLOGY: DICOTS
    16 Highly Efficient Transformation Protocol for Plum (Prunus domestica L.)
    17 Co-transformation of Grapevine Somatic Embryos to Produce Transgenic Plants Free of Marker Genes 201
    18 Initiation and Transformation of Grapevine Embryogenic Cultures 215
    19 Development of Highly Efficient Genetic Transfbrmation Protocols for Table Grape Sugraone and Crimson Seedless
    20 Cotton Pistil Drip Transformation Method
    21 Enhanced Agrobacterium-Mediated Transformation of Embryogenic Calli of Upland Cotton 245
    22 Targeted Biolistics for Improved Transfbrmation of Impatiens balsamina 255
    23 A Protocol for Transformation of Torenia
    24 Efficient Modification of Floral Traits by Heavy-Ion Beam Irradiation on Transgenic Torenia 275
    PART VI GENE TARGETING， SILENCING AND DIRECTED MUTATION
    25 Expression of Artificial MicroRNAs in Physcomitrella patens
    26 High Frequency of Single-Copy T-DNA Transformants Produced After Floral Dip in CRE-Expressing Arabidopsis Plants
    27 A Developmentally Regulated Cre-lox System to Generate Marker-Free Transgenic Brassica napus Plants
    28 Exploiting MultiSite Gateway and pENFRUIT Plasmid Collection for Fruit Genetic Engineering
    29 A One-Time Inducible Transposon to Create Knockout Mutants in Rice 369
    30 Marker-Free Gene Targeting by Recombinase-Mediated Cassette Exchange 379
    31 Targeting DNA to a Previously Integrated Transgenic Locus Using Zinc Finger Nucleases 391
    32 Double-Strand Break-Induced Targeted Mutagenesis in Plants 399
    PART VII METABOLIC ENGINEERING AND PHARMING
    33 Combinatorial Genetic Transformation of Cereals and the Creation of Metabolic Libraries for the Carotenoid Pathway 419
    34 Production of a His-Tagged Canecystatin in Transgenic Sugarcane 437
    35 Plastid Transformation as an Expression Tool for Plant-Derived Biopharmaceuticals
    36 Use of a Callus-Specific Selection System to Develop Transgenic Rice Seed Accumulating a High Level of Recombinant Protein
    PART VIII FIELD TESTS
    37 How to Grow Transgenic Arabidopsis in the Field.
    Index