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Mobile genetic elements : protocols and genomic applications / edited by Yves Bigot. — 2nd ed. — New York : Humana Press, c2012. – (58.17/M592/v.859)

Contents

    Contents
    
    Preface
    Contributors
    1 Transposable Elements as Tools for Reshaping the Genome: It Is a Huge World After All!
    2 Using and Understanding RepeatMasker
    3 Roadmap for Annotating Transposable Elements in Eukaryote Genomes
    4 To Detect and Analyze Sequence Repeats Whatever Be Their Origin...
    5 Exploring Bacterial Insertion Sequences with ISfinder: Objectives, Uses, and Future Developments
    6 Methods and Software in NGS for TE Analysis
    7 The Application of LTR Retrotransposons as Molecular Markers in Plants 115
    8 Individual Analysis of Transposon Polymorphisms by AFLP
    9 Construction of a Library of Random Mutants in the Spirochete Leptospira Biflexa Using a Mariner Transposon
    10 Ac-Ds Solutions for Rice Insertion Mutagenesis
    11 Engineering the Caenorhabditis elegans Genome by Mos1-Induced Transgene-Instructed Gene Conversion 189
    12 Genome-Wide Manipulations of Drosophila melanogaster with Transposons, Flp Recombinase, and ΦC31 Integrase 203
    13 The Sleeping Beauty Transposon Toolbox 229
    14 PiggyBac Toolbox
    15 Insertion Site Pattern: Global Approach by Linear Amplification-Mediated PCR and Mass Sequencing 255
    16 Comprehensive DNA Methylation Profiling of Human Repetitive DNA Elements Using an MeDIP-on-RepArray Assay
    17 Novel Approach for the Development of New Antibodies Directed Against Transposase-Derived Proteins Encoded by Human Neogenes 293
    Index