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Human pluripotent stem cells : methods and protocols / edited by Philip H. Schwartz, Robin L. Wesselschmidt. — New York : Humana Press, c2011. – (58.17/M592/v.767) |
Contents
Contents
PART I LABORATORY ESSENTIALS
1 The Stem Cell Laboratory: Design， Equipment， and Oversight
2 Stem Cell Banks: Preserving Cell Lines， Maintaining Genetic Integrity， and Advancing Research
PART II DERIVATION
3 Embryonic Stem Cell Derivation from Human Embryos
4 Derivation of Human Parthenogenetic Stem Cell Lines
5 Generation of Induced Pluripotent Stem Cell Lines from Human Fibroblasts via Retroviral Gene Transfer
6 Derivation of Induced Pluripotent Stem Cells by Lentiviral Transduction
7 Transgene-Free Production of Pluripotent Stem Cells Using piggyBac Transposons
PART III GROWTH， MAINTENANCE， AND EXPANSION
8 Traditional Human Embryonic Stem Cell Culture 107
9 Xeno-Free Culture of Human Pluripotent Stem Cells 125
10 Adaptation of Human Pluripotent Stem Cells to Feeder-Free Conditions in Chemically Defined Medium with Enzymatic Single-Cell Passaging 137
11 GMP Scale-Up and Banking of Pluripotent Stem Cells for Cellular Therapy Applications
12 Culture of Human Pluripotent Stem Cells on Glass Slides for High-Resolution Imaging 161
PART IV CHARACTERIZATION
13 Classical Cytogenetics: Karyotyping Techniques
14 FISH Analysis of Human Pluripotent Stem Cells
15 Immunocytochemical Analysis of Human Pluripotent Stem Cells
16 Flow Cytometric Analysis of Human Pluripotent Stem Cells
17 The Teratoma Assay: An In Vivo Assessment of Pluripotency
18 Detection of Copy Number Variation Using SNP Genotyping 243
19 Genome-Wide Epigenetic Analysis of Human Pluripotent Stem Cells by ChIP and ChIP-Seq
20 Basic Approaches to Gene Expression Analysis of Stem Cells by Microarrays 269
21 Human Pluripotent Stem Cells: The Development of High-Content Screening Strategies
22 Quantitative Proteome and Phosphoproteome Analysis of Human Pluripotent Stem Cells
PART V GENETIC MANIPULATION (GENE MODULATION)
23 Lentivirus-Mediated Modification of Pluripotent Stem Cells 315
24 Nucleofection of Human Embryonic Stem Cells 333
25 Nonviral Gene Delivery in Neural Progenitors Derived from Human Pluripotent Stem Cells 343
26 Gene Targeting in Human Pluripotent Stem Cells 355
27 Episomal Transgene Expression in Pluripotent Stem Cells 369
PART VI DIFFERENTIATION
28 The Generation of Embryoid Bodies from Feeder-Based or Feeder-Free Human Pluripotent Stem Cell Cultures
29 Derivation of Oligodendrocyte Progenitor Cells from Human Embryonic Stem Cells
30 Directed Differentiation of Dopamine Neurons from Human Pluripotent Stem Cells
31 Methods for the Derivation and Use of Cardiomyocytes from Human Pluripotent Stem Cells 419
32 In Vivo Evaluation of Putative Hematopoietic Stem Cells Derived from Human Pluripotent Stem Cells
33 Differentiation of Dendritic Cells from Human Embryonic Stem Cells
Index
