Protein microarrays : methods and protocols / edited by Ulrike Korf. — New York : Humana Press, c2011. – (58.17/M592/v.785)

Contents

    CONTENTS
    
    Preface
    Contributors
    PART I REVERSE PHASE PROTEIN ARRAYS
    1 Reverse Phase Protein Microarrays for Clinical Applications
    2 Impact of Blocking and Detection Chemistries on Antibody Performance for Reverse Phase Protein Arrays
    3 Phosphoprotein Stability in Clinical Tissue and Its Relevance for Reverse Phase Protein Microarray Technology
    4 Utilization of RNAi to Validate Antibodies for Reverse Phase Protein Arrays
    5 Antibody-Mediated Signal Amplification for Reverse Phase Protein Array-Based Protein Quantification
    6 Reverse-Phase Protein Lysate Microarray (RPA) for the Experimental Validation of Quantitative Protein Network Models
    7 Characterization of Kinase Inhibitors Using Reverse Phase Protein Arrays
    8 Use of Formalin-Fixed and Paraffin-Embedded Tissues for Diagnosis and Therapy in Routine Clinical Settings
    9 Producing Reverse Phase Protein Microarrays from Formalin Fixed Tissues 123
    10 Use of Reverse Phase Protein Microarrays to Study Protein Expression in Leukemia: Technical and Methodological Lessons Learned
    PART II ANTIBODY MICROARRAYS
    11 Antibody Microarrays as Tools for Biomarker Discovery
    12 Assessment of Antibody Specificity Using Suspension Bead Arrays
    13 Quantitative Analysis of Phosphoproteins Using Microspot Immunoassays 191
    14 Robust Protein Profiling with Complex Antibody Microarrays in a Dual-Colour Mode
    15 High-Throughput Studies of Protein Glycoforms Using Antibody-Lectin Sandwich Arrays
    16 Microspot Immunoassay-Based Analysis of Plasma Protein Profiles for Biomarker Discovery Strategies 237
    17 Recombinant Antibodies for the Generation of Antibody Arrays
    PART III PROTEIN MICROARRAYS
    18 Producing Protein Microarrays from DNA Microarrays
    19 Cell Arrays and High-Content Screening
    20 Probing Calmodulin Protein-Protein Interactions Using High-Content Protein Arrays
    21 Protein Function Microarrays for Customised Systems-Oriented Proteome Analysis
    22 Optimized Autoantibody Profiling on Protein Arrays
    PART IV SAMPLE IMMOBILIZATION STRATEGIES
    23 Inkjet Printing for the Production of Protein Microarrays..
    24 Impact of Substrates for Probe Immobilization
    25 Contact Printing of Protein Microarrays
    Index