In vitro neurotoxicology : methods and protocols / edited by Lucio G. Costa, Gennaro Giordano, Marina Guizzetti. — New York : Humana Press, c2011. – (58.17/M592/v.758)

Contents

    Contents
    
    1 In Vitro Neurotoxicology: An Introduction
    PART I MODEL SYSTEMS
    2 Primary Neurons in Culture and Neuronal Cell Lines for In Vitro Neurotoxicological Studies
    3 Determination of Metal Interactions with the Chaperone Hspa5 in Human Astrocytoma Cells and Rat Astrocyte Primary Cultures
    4 Microglia Cell Culture: A Primer for the Novice
    5 Neural Stem Cells for Developmental Neurotoxicity Studies..
    6 Preparation， Maintenance， and Use of Serum-Free Aggregating Brain Cell Cultures
    7 Neurospheres as a Model for Developmental Neurotoxicity Testing
    8 Acute Hippocampal Slice Preparation and Hippocampal Slice Cultures
    9 Glia-Neuron Sandwich Cocultures: An In Vitro Approach to Evaluate Cell-to-Cell Communication in Neuroinflammation and Neurotoxicity
    10 In Vitro Models to Study the Blood Brain Barrier
    PART II CELL DEATH
    11 Measurements of Cell Death in Neuronal and Glial Cells .
    12 Measurements of Neuronal Apoptosis..
    13 Measurement of Isoprostanes as Markers of Oxidative Stress
    14 Assessment of Glutathione Homeostasis 205
    15 Assessing Neuronal Bioenergetic Status 215
    PART III SIGNALING IN NEUROTOXICITY
    16 Fluorescent Assessment of Intracellular Calcium in Individual Cells 239
    17 Homeostatic Regulation of Glutamate Neurotransmission in Primary Neuronal Cultures
    18 Detection of Nitric Oxide Formation in Primary Neural Cells and Tissues 267
    19 JNK3-Mediatcd Apoptotic Cell Death in Primary Dopaminergic Neurons 279
    20 Measurement of Proteasomal Dysfunction in Cell Models of Dopaminergic Degeneration 293
    21 Cell Signaling and Neurotoxicity: Protein Kinase C In Vitro and In Vivo 307
    22 Cell Signaling and Neurotoxicity: 3H-Arachidonic Acid Release (Phospholipase A2) in Cerebellar Granule Neurons
    PART IV OTHER END-POINTS
    23 Quantitative Assessment of Neurite Outgrowth in PCI2 Cells
    24 Measurements of Astrocyte Proliferation
    25 Quantification of Synaptic Structure Formation in Cocultures of Astrocytes and Hippocampal Neurons
    26 Volume Measurements in Cultured Primary Astrocytes
    27 Assessment of Cholesterol Homeostasis in Astrocytes and Neurons
    28 Dose-Response or Dose-Effect Curves in In Vitro Experiments and Their Use to Study Combined Effects of Neurotoxicants
    Index