Plant kinases : methods and protocols / edited by Nico Dissmeyer, Arp Schnittger. — New York : Humana Press, c2011. – (58.17/M592/v.779)

Contents

    Contents
    
    Preface
    Contributors
    PART I OVERVIEW
    1 Guide to the Book Plant Kinases
    2 The Age of Protein Kinases
    PART II KINASE EXPRESSION， PURIFICATION AND ACTIVITY ASSAYS
    3 Expression and Purification of Active Protein Kinases from Wheat Germ Extracts
    4 Measurement of Plant Cyclin-Dependent Kinase Activity Using Immunoprecipitation-Coupled and Affinity Purification-Based Kinase Assays and the Baculovirus Expression System 65
    5 Mitogen-Activated Protein Kinase Activity and Reporter Gene Assays in Plants
    6 Use of Phospho-Site Substitutions to Analyze the Biological Relevance of Phosphorylation Events in Regulatory Networks 93
    7 Bacterial Assay to Study Plant Sensor Histidine Kinases 139
    8 Substrate Analysis of Arabidopsis PP2C-Type Protein Phosphatases
    PART III STUDYING KINASE FUNCTION AT THE CELLULAR LEVEL
    9 Modulating and Monitoring MAPK Activity During Programmed Cell Death in Pollen
    10 Sensitizing Plant Protein Kinases to Specific Inhibition by ATP-Competitive Molecules
    11 Fluorescence Fluctuation Analysis of Receptor Kinase Dimerization
    12 Quantifying Degradation Rates of Transmembrane Receptor Kinases
    13 Fluorescence Correlation Spectroscopy and Fluorescence Recovery After Photobleaching to Study Receptor Kinase Mobility In Planta 225
    PART IV IDENTIFICATION OF KINASE SUBSTRATES AND PHOSPHORYLATION-SITE DETECTION
    14 Bimolecular-Fluorescence Complementation Assay to Monitor Kinase-Substrate Interactions In Vivo 245
    15 Chemical Genetic Analysis of Protein Kinase Function in Plants
    16 Modified Metal-Oxide Affinity Enrichment Combined with 2D-PAGE and Analysis of Phosphoproteomes 273
    17 Phosphoproteomics Using iTRAQ
    Index 303