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Laser imaging and manipulation in cell biology / edited by Francesco S. Pavone. — Weinheim : Wiley-VCH, c2010. – (58.10565/L343) |
Contents
Contents
List of Contributors XI
Introduction ]
Part One Multiphoton Imaging and Nanoprocessing 7
1 Multiphoton Imaging and Nanoprocessing of Human Stem Cells
1.1 Introduction 9
1.2 Principle of Two-Photon Microscopy and Multiphoton Tomography 10
1.3 Multiphoton Microscopes and Multiphoton Tomographs 12
1.4 Endogenous Cellular Fluorophores and SHG Active Biomolecule Structures 14
1.5 Optical Nanoprocessing 17
1.6 Discussion and Conclusion 28
References 31
2 In Vivo Nanosurgery
2.1 Introduction 35
2.2 Physical Mechanisms 36
2.3 Experimental Setup 37
2.4 Subcellular Nanosurgery 38
2.5 In Vivo Nanosurgery 41
2.6 Conclusions 46
References 47
Part Two Light-Molecule Interaction Mechanisms 49
3 Interaction of Pulsed Light with Molecules: Photochemica and Photophysical Effects 51
3.1 Introduction 51
3.2 Basic Photophysics 52
3.3 Bleaching and Excited State Absorption 57
3.4 Multiphoton Absorption and Ionization 60
3.5 Relevance for Biomedical Applications 61
3.6 Conclusions 67
References 68
4 Chromophore-Assisted Light Inactivation: A Twenty-Year Retrospective 71
4.1 Historical Perspective 71
4.2 Family of CALI-Based Technologies 72
4.3 Spatial Restriction of Damage 73
4.4 Mechanism of CALI 74
4.5 Micro-CALI 75
4.6 Intracellular Targets of CALI 75
4.7 CALI In Vivo 76
4.8 High-Throughput Approaches 77
4.9 Future of CALI 77
References 78
5 Photoswitches 83
5.1 Introduction 83
5.2 Synthetic Photoswitches 84
5.3 Natural Photoswitches
References
6 Optical Stimulation of Neurons
6.1 Introduction
6.2 Neural Stimulation with Optical Radiation 100
6.3 Direct Optical Stimulation of Neural Tissue 103
References 108
Part Three Tissue Optical Imaging 113
7 Light-Tissue Interaction at Optical Clearing 115
7.1 Introduction 115
7.2 Light-Tissue Interaction 115
7.3 Tissue Clearing 120
7.4 Enhancers of Diffusion 130
7.5 Diffusion Coefficient Estimation 133
7.6 Applications of Tissue Optical Clearing to Different Diagnostic and Therapeutic Techniques 144
7.7 Conclusion 155
References 156
Part Four Laser Tissue Operation 165
8 Photodynamic Therapy - the Quest for Improved Dosimetry in the Management of Solid Tumors 167
8.1 Introduction 167
8.2 Photodynamic Reactions 168
8.3 Photosensitizers 173
8.4 PDT Dosimetry Models 177
8.5 Clinical Implementation 185
8.6 Where is PDT Heading? 188
References 193
9 Laser Welding of Biological Tissue: Mechanisms， Applications and Perspectives
9.1 Introduction 203
9.2 Mechanism of Thermal Laser Welding 206
9.3 Temperature Control in Laser Welding Procedures 211
9.4 Surgical Applications of Thermal Laser Welding 214
9.5 Future Perspectives 223
References 226
Conclusions 233
References 242
Index 243
