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Principles and techniques of biochemistry and molecular biology / edited by Keith Wilson and John Walker. — 7th ed. — Cambridge : Cambridge University Press, 2010. – (58.173/P957p/7th ed.) |
Contents
CONTENTS
Preface to the seventh edition
List of contributors
List of abbreviations
1 Basic principles
1.1 Biochemical and molecular biology studies
1.2 Units of measurement
1.3 Weak electrolytes
1.4 Quantitative biochemical measurements
1.5 Safety in the laboratory
1.6 Suggestions for further reading
2 Cell culture techniques
2.1 Introduction
2.2 The cell culture laboratory and equipment
2.3 Safety considerations in cell culture
2.4 Aseptic techniques and good cell culture practice
2.5 Types of animal cell， characteristics and maintenance in culture
2.6 Stem cell culture
2.7 Bacterial cell culture
2.8 Potential use of cell cultures
2.9 Suggestions for further reading
3 Centrifugation
3.1 Introduction
3.2 Basic principles of sedimentation
3.3 Types， care and safety aspects of centrifuges
3.4 Preparative centrifugation
3.5 Analytical centrifugation
3.6 Suggestions for further reading
4 Microscopy
4.1 Introduction
4.2 The light microscope
4.3 Optical sectioning
4.4 Imaging living cells and tissues
4.5 Measuring cellular dynamics
4.6 The electron microscope (EM)
4.7 Image archiving
4.8 Suggestions for further reading
5 Molecular biology， bioinformatics and basic techniques
5.1 Introduction
5.2 Structure of nucleic acids
5.3 Genes and genome complexity
5.4 Location and packaging of nucleic acids
5.5 Functions of nucleic acids
5.6 The manipulation of nucleic acids - basic tools and techniques
5.7 Isolation and separation of nucleic acids
5.8 Molecular biology and bioinformatics
5.9 Molecular analysis of nucleic acid sequences
5.10 The polymerase chain reaction (PCR)
5.11 Nucleotide sequencing of DNA
5.12 Suggestions for further reading
6 Recombinant DNA and genetic analysis
6.1 Introduction
6.2 Constructing gene libraries
6.3 Cloning vectors
6.4 Hybridisation and gene probes
6.5 Screening gene libraries
6.6 Applications of gene cloning
6.7 Expression of foreign genes
6.8 Analysing genes and gene expression
6.9 Analysing whole genomes
6.10 Pharmacogenomics
6.11 Molecular biotechnology and applications
6.12 Suggestions for further reading
7 Immunochemical techniques
7.1 Introduction
7.2 Making antibodies
7.3 Immunoassay formats
7.4 Immuno microscopy
7.5 Lateral flow devices
7.6 Epitope mapping
7.7 Immunoblotting
7.8 Fluorescent activated cell sorting (FACS)
7.9 Cell and tissue staining techniques
7.10 Immunocapture polymerase chain reaction (PCR)
7.11 Immunoafflnity chromatography (IAC)
7.12 Antibody-based biosensors
7.13 Therapeutic antibodies
7.14 The future uses of antibody technology
7.15 Suggestions for further reading
8 Protein structure， purification， characterization and function analysis
8.1 Ionic properties of amino acids and proteins
8.2 Protein structure
8.3 Protein purification
8.4 Protein structure determination
8.5 Proteomics and protein function
8.6 Suggestions for further reading
9 Mass spectrometric techniques
9.1 Introduction
9.2 Ionisation
9.3 Mass analysers
9.4 Detectors
9.5 Structural information by tandem mass spectrometry
9.6 Analysing protein complexes
9.7 Computing and database analysis
9.8 Suggestions for further reading
10 Electrophoretic techniques
10.1 General principles
10.2 Support media
10.3 Electrophoresis of proteins
10.4 Electrophoresis of nucleic acids
10.5 Capillary electrophoresis
10.6 Microchip electrophoresis
10.7 Suggestions for further reading
11 Chromatographic techniques
11.1 Principles of chromatography
11.2 Chromatographic performance parameters
11.3 High-performance liquid chromatography
11.4 Adsorption chromatography
11.5 Partition chromatography
11.6 Ion-exchange chromatography
11.7 Molecular (size) exclusion chromatography
11.8 Affinity chromatography
11.9 Gas chromatography
11.10 Suggestions for further reading
12 Spectroscopic techniques: I Spectrophotometric techniques
12.1 Introduction
12.2 Ultraviolet and visible light spectroscopy
12.3 Fluorescence spectroscopy
12.4 Luminometry
12.5 Circular dichroism spectroscopy
12.6 Light scattering
12.7 Atomic spectroscopy
12.8 Suggestions for further reading
13 Spectroscopic techniques: II Structure and interactions
13.1 Introduction
13.2 Infrared and Raman spectroscopy
13.3 Surface plasmon resonance
13.4 Electron paramagnetic resonance
13.5 Nuclear magnetic resonance
13.6 X-ray diffraction
13.7 Small-angle scattering
13.8 Suggestions for further reading
14 Radioisotope techniques
14.1 Why use a radioisotope?
14.2 The nature of radioactivity
14.3 Detection and measurement of radioactivity
14.4 Other practical aspects of counting of radioactivity and analysis of data
14.5 Safety aspects
14.6 Suggestions for further reading
15 Enzymes
15.1 Characteristics and nomenclature
15.2 Enzyme steady-state kinetics
15.3 Analytical methods for the study of enzyme reactions
15.4 Enzyme active sites and catalytic mechanisms
15.5 Control of enzyme activity
15.6 Suggestions for further reading
16 Principles of clinical biochemistry
16.1 Principles of clinical biochemical analysis
16.2 Clinical measurements and quality control
16.3 Examples of biochemical aids to clinical diagnosis
16.4 Suggestions for further reading
16.5 Acknowledgements
17 Cell membrane receptors and cell signalling
17.1 Receptors for cell signalling
17.2 Quantitative aspects of receptor-ligand binding
17.3 Ligand-binding and cell-signalling studies
17.4 Mechanisms of signal transduction
17.5 Receptor trafficking
17.6 Suggestions for further reading
18 Drug discovery and development
18.1 Human disease and drug therapy
18.2 Drug discovery
18.3 Drug development
18.4 Suggestions for further reading
Index
The colour figure section is between pages 128 and 129
