 |
DNA polymerases : discovery, characterization and functions in cellular DNA transactions / Ulrich Hübscher ... [et al.]. — Singapore : World Scientific, c2010. – (58.174252/D629) |
Contents
Contents
1. History and Discovery of DNA Polymerases
1.1 Discovering DNA: A First Step Towards Understanding the Basis of Life
1.2 Imaging an Enzyme that Assembles the Nucleotides into DNA
1.3 Late 1960s to Early 1970s: DNA Replication Shows Its Complexity
1.4 Concluding Remarks， Parts 1.1-1.3
1.5 Multiple DNA Polymerases in Eukaryotic Cells: DNA Polymerases α， β and γ as the First Ones
1.6 Early Attempts to Ascribe an in vivo Function to DNA Polymerases α， β and γ
1.7 DNA Polymerases δ and ε
1.8 1985: Polymerase Chain Reaction (PCR)， a Concept with Tremendous Practical Applications
1.9 Yeast DNA Polymerases
1.10 Plant Cell DNA Polymerases
1.11 Virus-Induced DNA Polymerases
1.12 1999-2000: Nucleotide Sequence Analysis of Eukaryotic Organisms Allowed the Identification of Many Novel Specialized DNA Polymerases
1.13 Concluding Remarks， Parts 1.5-1.12
References
2 DNA Polymerases in the Three Kingdoms of Life: Bacteria， Archaea and Eukaryotes
2.1 Synthesis and Maintenance of DNA in Nature Need DNA Polymerases 59
2.2 The DNA Polymerase Reaction 61
2.3 The Universal Structure of a DNA Polymerase Resembles a Human Right Hand 63
2.4 The Seven DNA Polymerase Families and Their Functions: An Overview 64
2.5 DNA Polymerase Holoenzymes 70
2.6 DNA Polymerases， Ring-Like Clamps and Clamp Loaders 73
2.7 DNA Polymerases， Alternative Clamps and Clamp Loaders 75
2.8 Replicative DNA Polymerases Interacting with Other Proteins 78
2.9 DNA Polymerases and the Single-Stranded DNA Binding Protein Replication Protein A 79
2.10 Chapter Summary 80
References 82
3 Structural and Functional Aspects of the Prokaryotic and Archaea DNA Polymerase Families
3.1 Escherichia coli
3.2 Bacillus subtilis
3.3 Other Bacteria
3.4 Archaea
3.5 Chapter Summary
References
4. Structural and Functional Aspects of the Eukaryotic DNA Polymerase Families
4.1 The High Number of Specialized Pathways in Eukaryotic Cells Requires a Plethora of Specialized DNA Synthesizing Enzymes
4.2 Eukaryotic DNA Polymerase Structure: The "Right Hand" of the Cell
4.3 Eukaryotic DNA Polymerases Accessory Subunits
4.4 Eukaryotic DNA Polymerase Fidelity: Structural and Functional Aspects
4.5 Biochemical and Functional Properties of the Different Eukaryotic DNA Polymerases
4.6 Interaction with Auxiliary Factors
4.7 Eukaryotic DNA Polymerases Are Tightly Regulated in the Cell Cycle
4.8 Chapter Summary
References
5. Global Functions of DNA Polymerases
5.1 Fifteen DNA Polymerases: Share of Workload and Redundancies
5.2 DNA Replication in Living Organisms Requires Three DNA Polymerase Molecules at the Replication Fork...
5.3 Different DNA Repair Pathways Have Their Own DNA Polymerases， But Can also Borrow Them from the Replication Machinery
5.4 Translesion DNA Synthesis in Eukaryotes Generally Requires Two DNA Polymerases: An Inserter and an Extender
5.5 Expression of DNA Polymerases
5.6 DNA Polymerases Switch between Different DNA Transactions
5.7 Functions of DNA Polymerases in Checkpoint Control .
5.8 Chapter Summary
References
6. Viral DNA Polymerases
6.1 Bacteriophage T4 DNA Polymerase
6.2 Bacteriophage T7 DNA Polymerase
6.3 HSV-1 DNA Polymerase
6.4 Protein Primed DNA Replication: Adenoviruses and Bacteriophage q~29
6.5 African Swine Virus DNA Polymerase
6.6 RNA-Dependent DNA Synthesis: Reverse Transcriptases
6.7 Chapter Summary
References
7. Synthetic Evolution of DNA Polymerases with Novel Properties
7.1 Why Design Enzymes with Novel Properties?
7.2 DNA Polymerases Have a Tight Active Site to Which the Substrates Fit
7.3 Methods to Evolve DNA Polymerases with Novel Properties 245
7.4 Applications of DNA Polymerases with Novel Properties 250
7.5 DNA Polymerases with Novel Properties 253
7.6 Chapter Summary
References
8. DNA Polymerases and Diseases
8.1 Introduction
8.2 DNA Polymerases and Genetic Stability
8.3 DNA Polymerases and Resistance to Chemotherapy..
8.4 DNA Polymerase y and Human Diseases
8.5 Chapter Summary
References
9. DNA Polymerases and Chemotherapy
9.1 DNA Polymerases Are Important Chemotherapeutic Targets
9.2 Strategies and Problems for the Design of Inhibitors of DNA Polymerases
9.3 Inhibitors of Herpesvirus DNA Replication
9.4 The Lack of Enantioselectivity of Viral and Human Enzymes and the L-Enantiomers of Nucleosides: The Dawn of a New Generation of Antiviral Drugs
9.5 Inhibitors of HIV-1 Reverse Transcriptase 301
9.6 Inhibitors of Hepatitis B DNA Polymerase.
9.7 Chapter Summary
References
