Inositol phosphates and lipids : methods and protocols / edited by Christopher J. Barker. — Totowa, N.J. : Humana, 2010. – (58.17/M592/v.645)

Contents

    Contents
    
    Preface
    Contributors
    1 The Role of Inositol and the Principles of Labelling， Extraction， and Analysis of Inositides in Mammalian Cells
    2 HPLC Separation of Inositol Polyphosphates
    3 Molecular Manipulation and Analysis of inositol Phosphate and Pyrophosphate Levels in Mammalian Cells
    4 A Femtomole-Sensitivity Mass Assay for Inositol Hexakisphosphate
    5 Synthesis of InsP7 by the Inositol Hexakisphosphate Kinase 1 (IP6K1)
    6 Protein Pyrophosphorylation by Diphosphoinositol Pentakisphosphate (InsP7)
    7 Synthesis and Nonradioactive Micro-analysis of Diphosphoinositol Phosphates by HPLC with Postcolumn Complexometry
    8 Diphosphosinositol Polyphosphates and Energy Metabolism Assay for ATP/ADP Ratio
    9 Isolation of Inositol 1，4，5-Trisphosphate Receptor-Associating Proteins and Selective Knockdown Using RNA Interference
    10 Phosphoinositide-Specific Phospholipase C β1 Signal Transduction in the Nucleus
    11 Methods to Assess Changes ill the Pattern of Nuclear Phosphoinositides 165
    12 Measurement of Phosphoinositide 3-Kinase Products in Cultured Mammalian Cells by HPLC
    13 Phosphoinositide Analysis by Liquid Chromatography-Mass Spectrometry 203
    14 Imaging Phosphoinositide Dynamics in Living Cells
    Index