Cellular programming and reprogramming : methods and protocols / edited by Sheng Ding. — New York, N.Y. : Humana Press, c2010. – (58.17/M592/v.636)

Contents

    Contents
    
    Contributors
    1 Human Embryonic Stem Cell Derivation， Maintenance， and Differentiation to Trophoblast
    2 Isolation and Maintenance of Mouse Epiblast Stem Cells
    3 Functional Assays for Hematopoietic Stem Cell Self-Renewal
    4 Isolation Procedure and Characterization of Multipotent Adult Progenitor Cells from Rat Bone Marrow
    5 Generation of Functional Insulin-Producing Cells from Human Embryonic Stem Cells In Vitro
    6 Mesoderm Cell Development from ES Cells
    7 Directed Differentiation of Red Blood Cells from Human Embryonic Stem Cells 105
    8 Directed Differentiation of Neural-stem cells and Subtype-Specific Neurons from hESCs
    9 Directing Human Embryonic Stem Cells to a
    10 Bovine Somatic Cell Nuclear Transfer
    11 Cell Fusion-Induced Reprogramming
    12 An Improved Method for Generating and Identifying Human Induced Pluripotent Stem Cells
    13 Using Small Molecules to Improve Generation of Induced Pluripotent Stem Cells from Somatic Cells
    14 Reprogramming of Committed Lymphoid Cells by Enforced Transcription Factor Expression 219
    15 Reprogramming of B Cells 233
    16 Adult Cell Fate Reprogramming: Converting Liver to Pancreas 251
    17 In Vitro Reprogramming of Pancreatic Cells to Hepatocytes 285
    18 Generation of Novel Rat and Human Pluripotent Stem Cells by Reprogramming and Chemical Approaches 293
    19 Small Molecule Screen in Zebrafish and HSC Expansion
    20 Zebrafish Small Molecule Screen in Reprogramming/Cell Fate Modulation
    Index 329