Membrane transporters in drug discovery and development : methods and protocols / edited by Qing Yan. — [Totowa, N.J.] : Humana Press, c2010. – (58.17/M592/v.637)

Contents

    Contents
    
    Preface
    Contributors
    1. Membrane Transporters and Drug Development: Relevance to Pharmacogenomics， Nutrigenomics， Epigenetics， and Systems Biology 1
    2. Bioinformatics for Transporter Pharmacogenomics and Systems Biology: Data Integration and Modeling with UML 23
    3. Multidrug Resistance: Phylogenetic Characterization of Superfamilies of Secondary Carriers that Include Drug Exporters 47
    4. Targeting Drug Transporters - Combining In Silico and In Vitro Approaches to Predict In Vivo 65
    5. Methods to Evaluate Transporter Activity in Cancer 105
    6. Analysis of Expression of Drug Resistance-Linked ABC Transporters in Cancer Cells by Quantitative RT-PCR 121
    7. Fluorescence Studies of Drug Binding and Translocation by Membrane Transporters 133
    8. A Model for Transport Studies of the Blood-Brain Barrier 149
    9. Genetic Variants in the Vesicular Monoamine Transporter 1 (VMAT1/SLC18A1) and Neuropsychiatric Disorders
    10. Equilibrium Binding and Transport by Vesicular Acetylcholine Transporter
    11. ABC Transporters in Ophthalmic Disease 221
    12. Imaging of Protein Translocation In Situ in Skeletal Muscle of Living Mice
    13. Glucose Transporters in Parasitic Protozoa 245
    14. NMR Studies of Membrane Proteins 263
    15. Site-Directed Mutagenesis in the Study of Membrane Transporters 277
    16. Xenopus laevis Oocytes 295
    17. Measurement of Intracellular pH 311
    18. Measurement of Plasma Membrane Calcium-Calmodulin-Dependent ATPase (PMCA) Activity 333
    19. Assessment of the Contribution of the Plasma Membrane Calcium ATPase， PMCA， Calcium Transporter to Synapse Function Using Patch Clamp Electrophysiology and Fast Calcium Imaging 343
    Subject Index 361