RNA interference : From biology to clinical applications / edited by Wei-Ping Min, Thomas Ichim. — New York : Springer, 2010. – (58.17/M592/v.623)

Contents

    Contents
    
    Contributors
    PART I PHYSIOLOGY OF RNA INTERFERENCE
    1 Endogenous Antiviral Mechanisms of RNA Interference A Comparative Biology Perspective
    2 Monitoring Innate Immune Recruitment by siRNAs in Mammalian Cells
    3 Current Knowledge of MicroRNAs and Noncoding RNAs in Virus-Infected Cells
    4 Allele-Specific Silencing by 1LNA Interference
    5 Computational siRNA Design Considering Alternative Splicing
    6 Bioinformatic Approaches to siRNA Selection and Optimization
    7 Optimized Gene Silencing by Co-expression of Multiple shRNAs in a Single Vector
    8 Strategies in Designing Multigene Expression Units to Downregulate HIV-1
    9 Designing Optimal siRNA Based on Target Site Accessibility 137
    10 Chemical Synthesis of2'-O-Alkylated siRNAs 155
    PART II RNA INTERFEILENCE IN THE LABORATORY AND siRNA DELIVERY
    11 siRNA Specific Delivery System for Targeting Dendritic Cells
    12 Hydrodynamic Delivery Protocols
    13 New Methods for Reverse Transfection with siRNA from a Solid Surface
    14 Nonviral siRNA Delivery for Gene Silencing in Neurodegenerative Diseases 211
    15 Using siRNA to uncover Novel oncogenic Signaling Pathways
    16 Biodegradable Polymer-Mediated sh/siRNA Delivery for cancer Studies
    17 Cellular siRNA Delivery Using TatUIA and Photo Induced RNA Interference
    18 Polyethylenimine (PEI)/siRNA Mediated Gene Knockdown In Vitro and In Vivo
    19 Transfection of siRNAs in Multiple Myeloma Cell lines
    PART III (，1.1NICAI. IMI'I.E*IENTATION
    20 A New Approach for Therapeutic use by RNA Interference in the Brain
    21 Inhibitory RNA Molecules ill Immunotherapy for Cancer
    22 Preventing Tissue Injury Using siRNA
    23 Preventing immune Rejection Through Gene Silencing
    24 Topical Application of siRNA Targeting Cutaneous dendritic Cells in Allergic Skin Disease
    25 Direct Application of siRNA for In Vivo Pain Research
    26 A Potential Therapeutic for Pandemic influenza Using RNA Interference
    27 Evaluation of Targets for Ovarian Cancer gene silencing therapy: In Vitro and In Vivo Approaches
    Index