Cell-free protein production : methods and protocols / edited by Yaeta Endo, Kazuyuki Takai and Takuya Ueda. — New York : Humana Press, c2010. – (58.17/M592/v.607)

Contents

    Contents
    
    1 Cell-Free Protein Preparation Through Prokaryotic Transcription-Translation Methods
    2 PURE Technology
    3 The Cell-Free Protein Synthesis System from Wheat Germ
    4 A Cell-Free Protein Synthesis System from Insect Cells
    5 Cell-Free Protein Synthesis Systems with Extracts from Cultured Human Cells
    6 Analysis of Protein Functions Through a Bacterial Cell-Free Protein Expression System
    7 Cell-Free-Based Protein Microarray Technology Using Agarose/DNA Microplate
    8 An Efficient Approach to the Production of Vaccines Against the Malaria Parasite
    9 Protein Engineering Accelerated by Cell-Free Technology
    10 Cell-Free Protein Production System with the E. coli Crude Extract for Determination of Protein Folds 101
    11 NMR Assignment Method for Amide Signals with Cell-Free Protein Synthesis System 113
    12 Cell-Free Protein Synthesis Technology in NMR High-Throughput Structure Determination 127
    13 Cell-Free Protein Synthesis for Structure Determination by X-ray Crystallography 149
    14 Production of Multi-Subunit Complexes on Liposome Through an E. coli Cell-Free Expression System 161
    15 Synthesis of a Hetero Subunit RNA Modification Enzyme by the Wheat Germ Cell-Free Translation System
    16 Strategies for the Cell-Free Expression of Membrane Proteins
    17 Production of Membrane Proteins Through the Wheat-Germ Cell-Free Technology
    18 Ribosome Display with the PURE Technology
    19 Incorporation of 3-Azidotyrosine into Proteins Through Engineering Yeast Tyrosyl-tRNA Synthetase and Its Application to Site-Selective Protein Modification 227
    20 Synthesis of Functional Proteins Within Liposomes 243
    Index