Cell-free protein production : methods and protocols / edited by Yaeta Endo, Kazuyuki Takai and Takuya Ueda. — New York : Humana Press, c2010. – (58.17/M592/v.607) |
Contents
Contents
1 Cell-Free Protein Preparation Through Prokaryotic Transcription-Translation Methods
2 PURE Technology
3 The Cell-Free Protein Synthesis System from Wheat Germ
4 A Cell-Free Protein Synthesis System from Insect Cells
5 Cell-Free Protein Synthesis Systems with Extracts from Cultured Human Cells
6 Analysis of Protein Functions Through a Bacterial Cell-Free Protein Expression System
7 Cell-Free-Based Protein Microarray Technology Using Agarose/DNA Microplate
8 An Efficient Approach to the Production of Vaccines Against the Malaria Parasite
9 Protein Engineering Accelerated by Cell-Free Technology
10 Cell-Free Protein Production System with the E. coli Crude Extract for Determination of Protein Folds 101
11 NMR Assignment Method for Amide Signals with Cell-Free Protein Synthesis System 113
12 Cell-Free Protein Synthesis Technology in NMR High-Throughput Structure Determination 127
13 Cell-Free Protein Synthesis for Structure Determination by X-ray Crystallography 149
14 Production of Multi-Subunit Complexes on Liposome Through an E. coli Cell-Free Expression System 161
15 Synthesis of a Hetero Subunit RNA Modification Enzyme by the Wheat Germ Cell-Free Translation System
16 Strategies for the Cell-Free Expression of Membrane Proteins
17 Production of Membrane Proteins Through the Wheat-Germ Cell-Free Technology
18 Ribosome Display with the PURE Technology
19 Incorporation of 3-Azidotyrosine into Proteins Through Engineering Yeast Tyrosyl-tRNA Synthetase and Its Application to Site-Selective Protein Modification 227
20 Synthesis of Functional Proteins Within Liposomes 243
Index