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Dendritic cell protocols / edited by Shalin H. Naik. — 2nd ed. — New York : Humana Press, c2010. – (58.17/M592/v.595) |
Contents
Contents
Foreword
Contributors
SECTION I REVIEWS
1. Review of Human DC Subtypes
2. Review of Murine Dendritic Cells: Types， Location， and Development 21
SECTION II HUMAN DC PROTOCOLS 43
3. Isolation of Human Blood DC Subtypes
4. Isolation of Immature Primary Langerhans Cells from Human Epidermal Skin 55
5. Isolation and In Vitro Generation of Gene-Manipulated Human Plasmacytoid and Conventional Dendritic Cells 67
6. In Vivo Modulation of Gene Expression by Lentiviral Transduction in "Human Immune System" Rag2-/-%-/- Mice
7. Generation of Stable Th1/CTL-， Th2-， and Thl7-Inducing Human Dendritic Cells
8. Pharmacological Modification of Dendritic Cells to Promote Their Tolerogenicity in Transplantation
9. Towards a Standardized Protocol for the Generation of Monocyte-Derived Dendritic Cell Vaccines 149
SECTION III MURINE DENDRITIC CELL METHODS 165
10. CD8+， CD8-， and Plasmacytoid Dendritic Cell Generation In Vitro Using fit3 Ligand 167
11. Generation of Large Numbers of Pro-DCs and Pre-DCs In Vitro 177
12. The Generation of Plasmacytoid and Conventional Dendritic Cells with M-CSF 187
13. Isolation of Common Dendritic Cell Progenitors (CDP) from Mouse Bone Marrow 195
14. The Isolation of Mouse Dendritic Cells from Lymphoid Tissues and the Identification of Dendritic Cell Subtypes by Multiparameter Flow Cytometry 205
15. Isolation of Cutaneous Dendritic Cells 231
16. Isolation of Skin Dendritic Cells from Mouse and Man 235
17. Identification and Isolation of Rodent Respiratory Tract Dendritic Cells 249
18. Isolation of Mouse Thymic Dendritic Cell Precursors 265
19. Isolation of Rat Intestinal Lymph DC 281
20. Mouse Models of Viral Infection: Influenza Infection in the Lung 299
21. DCs in Mouse Models of Intracellular Bacterial Infection 319
22. Studying the Function of Dendritic Cells in Mouse Models of Asthma 331
23. Direct Ex Vivo Activation of T cells for Analysis of Dendritic Cells Antigen Presentation 351
24. Methods to Study Pulmonary Dendritic Cell Migration
25. Measuring pH， ROS Production， Maturation， and Degradation in Dendritic Cell Phagosomes Using Cytofluorometry-Based Assays 383
26. Adaptive Treg Generation by DCs and Their Functional Analysis 403
27. Generation of Parabiotic Mice for the Study of DC and DC Precursor Circulation 413
28. Defining In Vivo Dendritic Cell Functions Using CD11c-DTR Transgenic Mice
Subject Index 443
