Plant microRNAs : methods and protocols / edited by Blake C. Meyers, Pamela J. Green. — Totowa, N.J : Humana, 2009. – (58.17/M592/v.592)

Contents

    Contents
    
    Preface
    Contributors
    1 Piecing the Puzzle Together: Genetic Requirements for miRNA Biogenesis in Arabidopsis thaliana
    2 Prediction of Plant miRNA Genes
    3 Methods for Isolation of Total RNA to Recover miRNAs and Other Small RNAs from Diverse Species
    4 miRNA Target Prediction ill Plants
    5 A Method to Discover Phased siRNA Loci
    6 Directed Gene Silencing with Artificial MicroRNAs
    7 Bioinformatics Analysis of Small RNAs in Plants Using Next Generation Sequencing Technologies
    8 High-Throughput Approaches for miRNA Expression Analysis
    9 In Situ Detection of miRNAs Using LNA Probes
    10 Analysis of miRNA Modifications
    11 MicroRNA Promoter Analysis
    12 Computational Methods for Comparative Analysis of Plant Small RNAs
    13 Biotic Stress-Associated microRNAs: Identification， Detection， Regulation， and Functional Analysis 183
    14 Abiotic Stress-Associated miRNAs: Detection and Functional Analysis 203
    15 Processing of miRNA Precursors 231
    16 Purification of Arabidopsis Argonaute Complexes and Associated Small RNAs 243
    17 Transient Assays for the Analysis of miRNA Processing and Function 255
    Index 265