Molecular endocrinology : methods and protocols / edited by Ok-Kyong Park-Sarge, Thomas E. Curry, Jr. — Totowa, N.J. : Humana, 2009. – (58.17/M592/v.590)

Contents

    Contents
    
    1. Determining the Affinity of Hormone-Receptor Interaction
    2. Determination of Serum Estradiol Levels by Radiometric and Chemiluminescent Techniques
    3. Identification of Natural Human Glucocorticoid Receptor (hGR) Mutations or Polymorphisms and Their Functional Consequences at the Hormone-Receptor Interaction Level
    4. Monitoring Insulin-Stimulated Production of Signaling Lipids at the Plasma Membrane
    5. Gene Expression Profiling in the Aging Ovary
    6. Genomics Analysis: Endometrium
    7. Detection of Ovarian Matrix Metalloproteinase mRNAs by In Situ Hybridization
    8. Adenoviral Gene Transfer into Isolated Pancreatic Islets
    9. Basic Molecular Techniques for the Detection of Single Nucleotide Polymorphisms: Genome-Wide Applications in Search for Endocrine Tumor Related Genes
    10. Methylated DNA Immunoprecipitation and Microarray-Based Analysis: Detection of DNA Methylation in Breast Cancer Cell Lines 165
    11. Use of Reporter Genes to Study the Activity of Promoters in Ovarian Granulosa Cells 177
    12. Use of Reporter Genes to Study Promoters of the Androgen Receptor 195
    13. Isolation of Proteins Associated with the DNA-Bound Estrogen Receptor ct 209
    14. Chromosome-Wide Analysis of Protein Binding and Modifications
    15. Genome-Wide Analysis for Protein-DNA Interaction: ChIP-Chip 235
    16. Detection ofERcz-SRC-1 Interactions Using Bioluminescent Resonance Energy Transfer 253
    17. Detection of Proteins Sumoylated In Vivo and In Vitro 265
    18. Identification of Alternative Transcripts Using Rapid Amplification of cDNA Ends (RACE) 279
    19. Use of Laser Capture Microdissection in Studying Hormone-Dependent Diseases: Endometriosis 295
    20. Reporter Mice for the Study of Intracellular Receptor Activity 307
    21. Real-Time Non-invasive Imaging of ES Cell-Derived Insulin Producing Cells 317
    22. Transgenic Mouse Technology: Principles and Methods 335
    23. Breast Tumor-Initiating Cells Isolated from Patient Core Biopsies for Study of Hormone Action 363
    24. Markers of Oxidative Stress and Sperm Chromatin Integrity
    25. Planning and Executing a Genome Wide Association Study (GWAS) 403
    Subject Index 419