Helicases : methods and protocols / edited by Mohamed M. Abdelhaleem. — New York, N.Y. : Humana Press, c2010. – (58.17/M592/v.587)

Contents

    Contents
    
    Preface
    Contributors
    1. Helicases: An Overview
    2. Fluorescent Biosensors to Investigate Helicase Activity
    3. Single-Molecule FRET Analysis of Helicase Functions
    4. Kinetics of Motor Protein Translocation on Single-Stranded DNA 45
    5. Experimental and Computational Analysis of DNA Unwinding and Polymerization Kinetics 57
    6. Protein Displacement by Helicases 85
    7. In Vivo Investigation of Protein-Protein Interactions for Helicases Using Tandem Affinity Purification 99
    8. Mapping Genomic Targets of DNA Helicases by Chromatin Immunoprecipitation in Saccharomyces cerevisiae
    9. Methods to Study How Replication Fork Helicases Unwind DNA 127
    10. Simple Enzymatic Assays for the In Vitro Motor Activity of Transcription Termination Factor Rho from Escherichia coli 137
    11. Single-Molecule Studies of RecBCD
    12. Mutational Analysis of Bloom Helicase
    13. In Vivo Analysis of Drosophila BLM Helicase Function During DNA Double-Strand Gap Repair
    14. Purification and Characterization of RecQ Helicases of Plants 195
    15. Fluorometric Assay of Hepatitis C Virus NS3 Helicase Activity 211
    16. A Method to Simultaneously Monitor Hepatitis C Virus NS3 Helicase and Protease Activities 223
    17. Computer Modeling of Helicases Using Elastic Network Model 235
    18. Duplex Unwinding with DEAD-Box Proteins
    19. Analysis of the RNA Helicase p68 (Ddx5) as a Transcriptional Regulator 265
    20. A Method to Study the Role of DDX3 RNA Helicase in HIV-1 281
    21. Molecular Characterization of Nuclear DNA Helicase II (RNA Helicase A) 291
    22. Regulation of Inter- and Intramolecular Interaction of RNA， DNA， and Proteins
    by MLE 303
    23. Biochemical Characterization of Human Upfl Helicase 327
    24. Assays of the Helicase， ATPase， and Exoribonuclease Activities of the Yeast Mitochondrial Degradosome 339
    25. Characterization of the Helicase Activity and Anti-telomerase Properties of Yeast Piflp In Vitro 359
    26. A Method to Confer Salinity Stress Tolerance to Plants by Helicase Overexpression 377
    27. A Method to Inhibit the Growth of Plasmodium falciparum by Double-Stranded RNA-Mediated Gene Silencing of Helicases 389
    Subject Index 401